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Mutation in the VP2 gene of P1-2A capsid protein increases the thermostability of virus-like particles of foot-and-mouth disease virus serotype O.

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Title Mutation in the VP2 gene of P1-2A capsid protein increases the thermostability of virus-like particles of foot-and-mouth disease virus serotype O.
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Creator Ganji VK
Biswal JK
Lalzampuia H
Basagoudanavar SH
Saravanan P
Tamil Selvan RP
Umapathi V
Reddy GR
Sanyal A
Dechamma HJ
 
Subject Differential scanning fluorescence
FMD
Thermostability
VLP
 
Description Not Available
Foot-and-mouth disease (FMD) is an economically important, global disease of cloven-hoofed animals. The conventional vaccine could bring down the incidence of disease in many parts of the world but has many limitations and in India, the disease is enzootic. More promisingly, the alternate vaccine candidates, virus-like particles (VLPs) are as immunogenic as a native virus but are more labile to heat than the live virus capsids. To produce stable VLPs, a single amino acid residue was mutated at 93 and 98 positions at VP2 inter-pentamer region of the P1-2A gene of FMD virus serotype O (IND/R2/75). The mutated capsid protein was expressed in insect cells and characterized for temperature and varying pH stability. Out of S93Y, S93F, S93C, S93H, and Y98F mutant, VLPs, S93Y, S93F, and Y98F showed improved stability at 37 °C for 75 days compared to wild capsid, which was evaluated by sandwich ELISA. Further, the stability analysis of purified VLPs either by differential scanning fluorescence (DSF) stability assay at different temperatures and pH conditions or by dissociation kinetics showed that the Y98F mutant VLPs were more stable than S93Y, S93F, S93C, and S93H mutant and wild-type VLPs. Immunization of guinea pigs with Y98F VLPs induced neutralizing antibodies and 60% of the animals were protected from the FMDV "O" 100 GPID50 challenge virus.
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Date 2023-05-10T04:18:22Z
2023-05-10T04:18:22Z
2018-08-22
 
Type Research Paper
 
Identifier Ganji VK, Biswal JK, Lalzampuia H, Basagoudanavar SH, Saravanan P, Tamil Selvan RP, Umapathi V, Reddy GR, Sanyal A, Dechamma HJ. Mutation in the VP2 gene of P1-2A capsid protein increases the thermostability of virus-like particles of foot-and-mouth disease virus serotype O. Appl Microbiol Biotechnol. 2018 Oct;102(20):8883-8893. doi: 10.1007/s00253-018-9278-9. Epub 2018 Aug 22. PMID: 30136205.
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http://krishi.icar.gov.in/jspui/handle/123456789/77001
 
Language English
 
Relation Not Available;
 
Publisher SPRINGER