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Expression of foot-and-mouth disease virus non-structural protein 3A upregulates the expression of autophagy and immune response genes in vitro.

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Title Expression of foot-and-mouth disease virus non-structural protein 3A upregulates the expression of autophagy and immune response genes in vitro.
Not Available
 
Creator Lalzampuia, H
Vishweshwar Kumar Ganji
Subhadra Elango
Narayanan Krishnaswamy
V. Umapathi
Golla Ramalinga Reddy
Aniket Sanyal
H. J. Dechamma
 
Subject FMD
3A protein
BHK-21
Autophagy
Immune response
 
Description Not Available
Foot-and-mouth disease (FMD) virus 3A protein regulates viral replication and virulence; thus, we generated BHK-Flp-In cell line expressing 3A protein because it can serve as helper cell line for infecting a replication defective FMDV to produce a live disabled vaccine. FMDV Asia1 3A was amplified, cloned in pcDNA vector and confirmed by sequencing. The 3A gene was subcloned in pcEF/FRT vector and transfected in BHK-Flp-In cells and transformed cells were selected by resistance to hygromycin and susceptibility to zeocin antibiotics. The BHK-Flp-In cells expressing 3A protein was designated as Flp-In3A. Western blot and immunofluorescence confirmed that Flp-In3A cells expressed FMDV3A protein. Absolute quantitation of 3A transcripts showed peak expression at 6 h in Flp-In3A cells followed by a sharp decrease and the cells showed growth retardation for 2 h post-seeding with cytoplasmic vacuolations with advancing time. Response to infection with FMDV Asia1 virus revealed smaller plaques in Flp-In3A cells. Then, we investigated the effect of FMDV3A expression on autophagy related genes by real time PCR. Most autophagy genes were upregulated by 9 h post-seeding of which, autophagosome marker LC3B-II was demonstrated by western blot. Transient expression of 3A in PK-15 cells upregulated both Th1 and Th2 genes. The study suggested that the expressed 3A protein of FMDV cannot be used for 3A trans-supplementation in helper cells; however, it acts as an endogenously processed antigen that has the potential to elicit immune response in vivo.
Not Available
 
Date 2023-05-10T04:28:50Z
2023-05-10T04:28:50Z
2021-01-15
 
Type Research Paper
 
Identifier H L, Kumar Ganji V, Elango S, Krishnaswamy N, V U, Reddy GR, Sanyal A, Hj D. Expression of foot-and-mouth disease virus non-structural protein 3A upregulates the expression of autophagy and immune response genes in vitro. Virus Res. 2021 Jan 15;292:198247. doi: 10.1016/j.virusres.2020.198247. Epub 2020 Nov 27. PMID: 33253718.
0168-1702
http://krishi.icar.gov.in/jspui/handle/123456789/77021
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER