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Analysis of the matrix protein gene sequence of the Asian lineage of peste-des-petits ruminants vaccine virus.

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Title Analysis of the matrix protein gene sequence of the Asian lineage of peste-des-petits ruminants vaccine virus.
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Creator D Muthuchelvan
A Sanyal
B P Sreenivasa
P Saravanan
P Dhar
R P Singh
R K Singh
S K Bandyopadhyay
 
Subject M gene
peste-des-petits ruminants vaccine virus
nucleotide sequence
 
Description Not Available
The M gene nucleotide sequence of an Indian peste-des-petits ruminants (PPRV) vaccine virus (“PPRV Sungri/96”) belonging to Asian lineage was determined. The gene is 1476 nucleotides long with a single open reading frame (ORF). The nucleotide and predicted amino acid sequence was compared with the homologous region of the African Lineage Vaccine virus “PPRV/Nigeria/75/1”. The nucleotide sequence of the “PPRV Sungri/96” was 86% identical to that of “PPRV/Nigeria/75/1”, while a homology of 93% and 95% could be observed in the ORF and amino acids level, respectively. The M gene encodes a protein of 335 amino acids, with a predicted molecular weight (MW) of 37.8 kDa. The ORF is flanked by a 3′ untranslated region of 436 nucleotides and a high level of sequence divergence (∼30%) could be observed in this region between the vaccine viruses of Asian and African lineages. A high degree of conservation of several amino acids of this protein observed previously was also confirmed in this study.
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Date 2023-05-10T04:28:01Z
2023-05-10T04:28:01Z
2006-03-10
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77018
 
Language English
 
Relation Not Available;
 
Publisher Veterinary microbiology