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Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.

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Title Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.
Not Available
 
Creator Renjith Sebastian
M. Sravanthi
V. Umapathi
N Krishnaswamy
M. Priyanka
H.J. Dechamma
K. Ganesh
Suresh H. Basagoudanavar
A. Sanyal
G.R. Reddy
 
Subject FMD
Virus
Macrophage
Polarization
Cytokines
Antiviral genes
 
Description Not Available
Despite the fact that macrophages link the innate and adaptive arms of immunity, it’s role in the early infection of foot and mouth disease virus (FMDV) is largely unknown. Recently, depletion of macrophages in vivo after vaccination has shown to drastically diminish the protection against FMDV challenge in mouse model. Even the ability of macrophages to reduce or resist FMDV infection is not known hitherto. Therefore, we examined the replication ability of FMDV in mice peritoneal macrophages and the responsiveness in terms of macrophage polarization and cytokine production. Negative strand specific RT-PCR indicated replication of FMDV RNA in macrophages. Absolute quantitation of FMDV transcripts, immunofluorescence studies and titre of the infectious progeny virus revealed that replication peaked at 12 hpi and significantly declined by 18 hpi indicating non-progressive replication in the infected macrophages. Further, significant up regulation of inducible nitric oxide synthase by 8 –12 hpi and increase of M1 specific CD11c + cells by 42.6 % after infection showed that FMDV induce M1 polarization. A significant up regulation of TNFα and IL12 transcripts at 8 hpi supported that M1 macrophages were functional. Further, we studied the expression of Type I to III interferons (IFN) and other antiviral molecules. The results indicate a marked up regulation of Type I IFNα and β by 9.2 and 11.2 fold, respectively at 8 hpi. Of the four IFN stimulated genes (ISG), viperin showed a significant up regulation by 286-fold at 12 hpi in the mice macrophages. In conclusion, the results suggest that replication of FMDV in mice peritoneal macrophages is non-progressive with up regulation of Type I IFN and ISGs. Further, FMDV induces M1 polarization in murine peritoneal macrophages.
Not Available
 
Date 2023-05-10T04:32:14Z
2023-05-10T04:32:14Z
2020-05-01
 
Type Research Paper
 
Identifier Sebastian R, Sravanthi M, Umapathi V, Krishnaswamy N, Priyanka M, Dechamma HJ, Ganesh K, Basagoudanavar SH, Sanyal A, Reddy GR. Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization. Virus Res. 2020 May;281:197906. doi: 10.1016/j.virusres.2020.197906. Epub 2020 Feb 25. PMID: 32109526; PMCID: PMC7114663.
0168-1702
http://krishi.icar.gov.in/jspui/handle/123456789/77029
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER