Record Details

Expression of foot-and-mouth disease virus non-structural protein, 3D in insect cells and its application in detection of anti-FMDV antibodies.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Expression of foot-and-mouth disease virus non-structural protein, 3D in insect cells and its application in detection of anti-FMDV antibodies.
Not Available
 
Creator Rakesh Kumar
M Hosamani
B P Sreenivasa
Anil Kotyal
R Venkataramanan
 
Subject Non-structural proteins
foot-and-mouth disease
viral activity
 
Description Not Available
Non-structural proteins (NSPs) based diagnostics are useful for large-scale sero-surveillance of foot-and-mouth disease (FMD) and to monitor viral activity as a follow up to the vaccination campaign in FMD endemic countries like India which aim at disease control through vaccination. These diagnostics are also handy in the serology of import/export of cloven-footed animals. In the present study, non-structural protein RNA polymerase (3D gene) of FMD virus (FMDV) was expressed using baculovirus expression system. Protein expression was analyzed by SDS-PAGE and confirmed by its immuno-reactivity with serum from a FMDV infected bovine, in the western blot. Recombinant 3D protein was purified and evaluated in the indirect ELISA with 1072 cattle serum samples. Diagnostic sensitivity and specificity of the assay were found to be 92 and 100 %, respectively, when tested with cattle sera of known FMD status. The 3D based ELISA developed here is useful for screening the animals as an adjunct to other NSP based diagnostics available for routine serosurveillance of FMD.
Not Available
 
Date 2023-05-10T04:41:48Z
2023-05-10T04:41:48Z
2012-09-06
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77041
 
Language English
 
Relation Not Available;
 
Publisher Indian journal of virology