Rescue of infective virus from a genome- length cDNA clone of the FMDV serotype O (IND-R2/75) vaccine strain and its characterization.
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Title |
Rescue of infective virus from a genome- length cDNA clone of the FMDV serotype O (IND-R2/75) vaccine strain and its characterization.
Not Available |
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Creator |
R Rajasekhar
M Hosamani S H Basagoudanavar B P Sreenivasa R P Tamil Selvan P Saravanan R Venkataramanan |
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Subject |
foot and mouth disease virus
infectious cDNA Viral genome |
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Description |
Not Available
We report here the construction and characterization of an infectious cDNA clone of the Indian vaccine strain of foot and mouth disease virus (FMDV) serotype O, IND-R2/75. Viral genome was amplified by reverse transcription-polymerase chain reaction (RT-PCR) in five fragments and subsequently assembled sequentially in a plasmid vector to generate a complete cDNA clone, flanked by the T7 RNA polymerase promoter and poly (A) tail at 5′ and 3′ ends, respectively. Transfection of BHK-21 cells with the RNA transcribed from this genome-length cDNA construct allowed the recovery of infectious recombinant FMDV particles as evidenced by cytopathic effect in BHK-21 cells. Characterization of the recombinant virus revealed its similarity to the parental strain. Recombinant virus could be distinguished from the parental virus based on the presence of a unique marker sequence in the former, which was incorporated in the cDNA using a silent mutation. The virus showed no significant amino acid changes in the capsid-coding region when serially passaged up to ten times in BHK-21 cells, while retaining the marker sequence. Not Available |
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Date |
2023-05-10T10:08:01Z
2023-05-10T10:08:01Z 2013-08-01 |
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Type |
Article
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77059 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Research in Veterinary Science
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