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Rescue of infective virus from a genome- length cDNA clone of the FMDV serotype O (IND-R2/75) vaccine strain and its characterization.

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Title Rescue of infective virus from a genome- length cDNA clone of the FMDV serotype O (IND-R2/75) vaccine strain and its characterization.
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Creator R Rajasekhar
M Hosamani
S H Basagoudanavar
B P Sreenivasa
R P Tamil Selvan
P Saravanan
R Venkataramanan
 
Subject foot and mouth disease virus
infectious cDNA
Viral genome
 
Description Not Available
We report here the construction and characterization of an infectious cDNA clone of the Indian vaccine strain of foot and mouth disease virus (FMDV) serotype O, IND-R2/75. Viral genome was amplified by reverse transcription-polymerase chain reaction (RT-PCR) in five fragments and subsequently assembled sequentially in a plasmid vector to generate a complete cDNA clone, flanked by the T7 RNA polymerase promoter and poly (A) tail at 5′ and 3′ ends, respectively. Transfection of BHK-21 cells with the RNA transcribed from this genome-length cDNA construct allowed the recovery of infectious recombinant FMDV particles as evidenced by cytopathic effect in BHK-21 cells. Characterization of the recombinant virus revealed its similarity to the parental strain. Recombinant virus could be distinguished from the parental virus based on the presence of a unique marker sequence in the former, which was incorporated in the cDNA using a silent mutation. The virus showed no significant amino acid changes in the capsid-coding region when serially passaged up to ten times in BHK-21 cells, while retaining the marker sequence.
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Date 2023-05-10T10:08:01Z
2023-05-10T10:08:01Z
2013-08-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77059
 
Language English
 
Relation Not Available;
 
Publisher Research in Veterinary Science