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Establishment of aseptic culture in banana cv Rajapuri Bale (AAB) through male flower culture

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Title Establishment of aseptic culture in banana cv Rajapuri Bale (AAB) through male flower culture
 
Creator Habibi, Ajmal
Guranna, Prabhuling
 
Subject Benzylaminopurine
thidiazuran
coconut water
male flower
greening and bulging
aseptic culture
 
Description 396-403
Rajapuri Bale (AAB) is a popular cultivar of banana grown in northern parts of Karnataka especially in Bagalkote,
Vijaypur and Belgaum districts. The demand for planting material of this cultivar is high and tissue cultured plantlets are not
available because of the inherent problem in the initial establishment of the culture. The present investigation was carried out to
optimize protocol for establishment of aseptic culture for facilitating large scale multiplication. Different types and levels of
cytokinins viz., benzylaminopurine (BAP), thidiazuran (TDZ) and varying sizes of male flower explants were tested. Among the
different cytokinin treatments, Murashige and Skoog (MS) basal medium enriched with 5 mg/l BAP along with 1 mg/l
α-naphthalene acetic acid (NAA) and 150 ml/l coconut water recorded the maximum establishment of aseptic cultures
(100.00%), low browning (+) and maximum greening and bulging (+++). Among different explant size tested, significantly
maximum establishment of aseptic culture (100%), low browning (+) and maximum greening and bulging (+++) were observed
with male flower explant size of 3 cm.
 
Date 2023-05-09T11:14:03Z
2023-05-09T11:14:03Z
2023-05
 
Type Article
 
Identifier 0972-5849 (print) 0975-0967 (online)
http://nopr.niscpr.res.in/handle/123456789/61875
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(4) [October 2021]