Regeneration of plantlets through protocorm-like bodies: An alternative method for vanilla micro propagation
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Title |
Regeneration of plantlets through protocorm-like bodies: An alternative method for vanilla micro propagation
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Creator |
Malhotra, Era Vaidya
Bansal, Sangita Pathania, Pooja Mali, Suresh Chand Agrawal, Anuradha |
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Subject |
Genetic fidelity
histology PLBs shoot regeneration vanilla |
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Description |
388-395
An efficient protocol for direct plant regeneration inclusive of rooting and genetic fidelity testing via protocorm-like body (PLB) induction on nodal segments of Vanilla planifolia is presented. Effects of 16 different combinations of plant growth regulators, namely, benzyl aminopurine (BAP), zeatin and naphthalene acetic acid (NAA) were evaluated for PLB induction. BAP at a concentration of 1.5 mg/l together with 2 mg/l NAA in Murashige and Skoog (MS) medium was the most effective in inducing PLBs. Induced PLBs showed good proliferation on peptone supplemented media. Histological observation revealed the sub dermal origin of PLBs, gradually forming shoot apical meristem, shoot primordia and leaf primordia. Mature PLBs regenerated to form shoots on 20 media combinations tested for plantlet regeneration. Best induction rate of 5.08 shoots per PLB after eight weeks of culture was observed on MS medium containing 1 mg/l BAP and 1.5 mg/l NAA. NAA had a stimulatory effect on in vitro rooting with best response recorded on MS medium supplemented with 1 mg/l NAA. Genetic fidelity analysis of well-developed plantlets using inter sequence simple repeat (ISSR) markers revealed no change amongst the tested plants and their mother stocks. Direct regeneration of plantlets by induction of PLBs is an efficient alternative to routine micro-propagation via shoot tips/nodal explants for mass multiplication and germplasm conservation of this commercially important orchid species. |
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Date |
2023-05-09T11:16:53Z
2023-05-09T11:16:53Z 2023-05 |
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Type |
Article
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Identifier |
0972-5849 (print) 0975-0967 (online)
http://nopr.niscpr.res.in/handle/123456789/61876 |
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Language |
en
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Publisher |
NIScPR-CSIR, India
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Source |
IJBT Vol.20(4) [October 2021]
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