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Regeneration of plantlets through protocorm-like bodies: An alternative method for vanilla micro propagation

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Title Regeneration of plantlets through protocorm-like bodies: An alternative method for vanilla micro propagation
 
Creator Malhotra, Era Vaidya
Bansal, Sangita
Pathania, Pooja
Mali, Suresh Chand
Agrawal, Anuradha
 
Subject Genetic fidelity
histology
PLBs
shoot regeneration
vanilla
 
Description 388-395
An efficient protocol for direct plant regeneration inclusive of rooting and genetic fidelity testing via protocorm-like
body (PLB) induction on nodal segments of Vanilla planifolia is presented. Effects of 16 different combinations of plant
growth regulators, namely, benzyl aminopurine (BAP), zeatin and naphthalene acetic acid (NAA) were evaluated for PLB
induction. BAP at a concentration of 1.5 mg/l together with 2 mg/l NAA in Murashige and Skoog (MS) medium was the
most effective in inducing PLBs. Induced PLBs showed good proliferation on peptone supplemented media. Histological
observation revealed the sub dermal origin of PLBs, gradually forming shoot apical meristem, shoot primordia and leaf
primordia. Mature PLBs regenerated to form shoots on 20 media combinations tested for plantlet regeneration. Best
induction rate of 5.08 shoots per PLB after eight weeks of culture was observed on MS medium containing 1 mg/l BAP and
1.5 mg/l NAA. NAA had a stimulatory effect on in vitro rooting with best response recorded on MS medium supplemented
with 1 mg/l NAA. Genetic fidelity analysis of well-developed plantlets using inter sequence simple repeat (ISSR) markers
revealed no change amongst the tested plants and their mother stocks. Direct regeneration of plantlets by induction of PLBs
is an efficient alternative to routine micro-propagation via shoot tips/nodal explants for mass multiplication and germplasm
conservation of this commercially important orchid species.
 
Date 2023-05-09T11:16:53Z
2023-05-09T11:16:53Z
2023-05
 
Type Article
 
Identifier 0972-5849 (print) 0975-0967 (online)
http://nopr.niscpr.res.in/handle/123456789/61876
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(4) [October 2021]