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Nuclear localization and target genes analysis of an ABA and drought-responsive transcription factor (RDA1) in rice

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Title Nuclear localization and target genes analysis of an ABA and drought-responsive transcription factor (RDA1) in rice
 
Creator Kumar, Amresh
Kumar, Vaibhav
Maheshwari, Chirag
Garg, Nitin Kumar
Mawlong, Ibandalin
Tyagi, Aruna
 
Subject Abiotic stress
AP2-ERF
transcription factor
transient expression
water deficit stress
 
Description 364-374
Apetala2/Ethylene Responsive Fector (AP2/ERF) family transcription factors (TFs) play an important role in controlling
cellular processes and regulating important functions of plant growth and development as well as responses to
environmental stimuli. AP2/ERF transcription factor responsive to drought and ABA (RDA1) from rice genotype N22 was
chosen for this study. Transient expression revealed that RDA1 was localized in the nucleus. Quantitative expression studies
of RDA1 under three different treatments namely drought, ABA and drought + ABA at three different stress levels showed
maximum expression (3.92 fold) under drought +ABA treatment. Further, higher expression was found in N22 (drought
tolerant) compared to IR 64 (susceptible) at different stress levels. Temporal expression revealed higher relative expression
in leaf tissue compared to root tissue in both cultivars. RDA1 acts as a cytokinin primary response gene as per in silico
characterization and many of its target genes are involved in transcriptional, post-transcriptional and translational regulation
of protein biosynthesis. Relative co-expression studies for target genes revealed positive co-expression of GAMYB-binding
protein, leucyl tRNA synthetase and negative co-expression of ribosomal protein L7, crooked neck (CRN) protein and XPAbinding
protein with RDA1. Differential co-expression of WD40 and DUF382 domain protein was observed in root and leaf
tissue with RDA1.
 
Date 2023-05-09T11:26:57Z
2023-05-09T11:26:57Z
2023-05
 
Type Article
 
Identifier 0972-5849 (print) 0975-0967 (online)
http://nopr.niscpr.res.in/handle/123456789/61879
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(4) [October 2021]