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Isolation of a thermophilic Meiothermus sp. strain JE2 and its infective phage vB_Msp_JE2 from Rajwadi hot spring, India

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Title Isolation of a thermophilic Meiothermus sp. strain JE2 and its infective phage vB_Msp_JE2 from Rajwadi hot spring, India
 
Creator Faria, Nicola
Ghadi, Sanjeev C.
 
Subject Hot spring
thermophilic bacteria
bacteriophage
Meiothermus sp
 
Description 355-363
A thermophilic, red-pigmented aerobic bacteria designated as strain JE2 was isolated from the water sample of the
Rajwadi hot spring. Bacterial strain JE2 was Gram-negative, rod-shaped that grew optimally at pH 7.5 and 60°C. Molecular
identification of the 16S rRNA sequence indicated that bacterial strain JE2 formed a coherent cluster with genus
Meiothermus. Subsequently, a lytic bacteriophage designated as vB_Msp_JE2 was isolated from the Rajwadi hot spring that
could be propagated using Meiothermus sp. strain JE2. One-step growth curve using Meiothermus sp. strain JE2 as
host demonstrated a latent period of 70 min and a burst size of 20. Bacteriophage vB_Msp_JE2 was stable at pH 6.0 - 8.0.
Additionally, the phage was thermostable at 60°C and was completely inactivated above 75°C. Since, most microbial studies
have been reported predominantly from the Himalayan geothermal province, the present study reports the association of a
unique culturable pigmented thermophilic bacterial host and bacteriophage from Rajwadi hot spring present in the West
coast geothermal province, India.
 
Date 2023-05-09T11:28:59Z
2023-05-09T11:28:59Z
2023-04
 
Type Article
 
Identifier 0972-5849 (print) 0975-0967 (online)
http://nopr.niscpr.res.in/handle/123456789/61880
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(4) [October 2021]