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Inhibition of biofilm growth of Gram-positive and Gram-negative bacteria on tuned polyurethane nanofibers

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Title Inhibition of biofilm growth of Gram-positive and Gram-negative bacteria on tuned polyurethane nanofibers
 
Creator Khan, Rumysa Saleem
Wani, Taha Umair
Rather, Anjum Hamid
Rafiq, Muheeb
Sheikh, Faheem A
 
Subject Antibacterial
Biofilm
Electrospinning
Nanofiber
Nanoparticles
Silver
 
Description 112-121
Biofilm formation is a process of bacterial attachment whereby they fasten irreversibly to a biomaterial surface and lead to
unwanted phenotypic changes. The chief concern is its formation and to prevent the harmful changes that follow the
accumulation of bacteria on implants, so the scientific community has made efforts. In this study, we attempted to fabricate a
novel tissue engineering candidate to prevent the biofilm formation desired by ideal biomaterials. We prepared the
micro/nanofibers of polyurethane (PU) incorporated with hydrophilic β-cyclodextrin (CD) by electrospinning technique.
Further on, these as-spun fibers were in fused with an antibacterial agent. As an antibacterial agent, silver nanoparticles
(Ag NPs) were adsorbed on scaffolds. Among the varied methods of its adsorption, adsorption by sonication and hydrothermal
process were chosen. Characterization studies performed were scanning electron microscopy (SEM) and water contact angle
analysis. The uniform morphology of nanofibers was seen in SEM micrographs which mimics the extracellular matrix. The
hydrophilicity test showed the increased hydrophilicity of scaffolds with a decrease in contact angle in CD and Ag NPs
incorporated fibre scaffolds. The Ag release assay showed slow release in the case of the fibers where Ag was adsorbed by
hydrothermal treatment compared to adsorption by sonication. The antibacterial tests show inhibition of bacteria to different
degrees by the fibers. The highest zones were seen in the case of samples with Ag NPs adsorption by sonication. The in vitro
MTT assay presented that these scaffolds were non-toxic to the cells and could be employed in biological applications.
 
Date 2023-05-11T09:42:36Z
2023-05-11T09:42:36Z
2023-05
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/61894
https://doi.org/10.56042/ijnpr.v14i1.1133
 
Language en
 
Relation A61P 31/00
A61P 31/04
 
Publisher NIScPR-CSIR,India
 
Source IJNPR Vol.13(4) [March 2023]