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Different extraction methods for Thymoquinone from Nigella sativa L. seeds and antioxidant activity

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Title Different extraction methods for Thymoquinone from Nigella sativa L. seeds and antioxidant activity
 
Creator Chung, Kah Xin
Wei, Patricia Lee Yee
Akowuah, Gabriel Akyirem
 
Subject Black seed
DPPH Assay
Extraction methods
Free radical scavenging activity
Thymoquinone
UV-Vis Spectroscopy
 
Description 75-80
The study aimed to investigate the effects of different extraction methods on the content of thymoquinone using UV-Vis
spectroscopy and the antioxidant activities of thymoquinone by measuring the free radical scavenging activity. One gram of
powdered Nigella sativa L. black seed was added to 20 mL of solvents (hexane and methanol) into a conical flask and
mixed. The mixtures were extracted by different methods which were maceration, percolation, and ultrasonic-assisted
extraction. In the percolation method, the mixtures were placed in a water bath of 40°C for 4 h whereas, for maceration, the
mixtures were left at room temperature for 4 h. On the other hand, the mixtures were replaced in an ultrasonic bath at room
temperature for 2 h in ultrasonic-assisted extraction. The extracts were centrifuged at 4000 rpm at 4°C for 10 min and
filtered. The extracts were then analyzed qualitatively and quantitatively by a validated method (UV-Vis spectroscopy). On
the other hand, the antioxidant activity was measured by DPPH radical scavenging. In UV analysis, the percolation method
with methanol extracted the most content (0.9102%) of thymoquinone. The free radical scavenging activity was observed
highest (95.6891%) in the methanol extracts by percolation method. The results in the present study show that methanol
extract by percolation method is the best method to extract the main bioactive component, thymoquinone from N. sativa
seed. Furthermore, the N. sativa seed extracts exhibit antioxidant activity.
 
Date 2023-05-11T10:02:28Z
2023-05-11T10:02:28Z
2023-05
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/61899
https://doi.org/10.56042/ijnpr.v14i1.1134
 
Language en
 
Relation A61K 36/00
A61K 131/00
A61P 39/00
 
Publisher NIScPR-CSIR,India
 
Source IJNPR Vol.13(4) [March 2023]