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Antioxidant potential and in vitro cytotoxicity study of Saraca indica extract on lead-induced toxicity in HepG2 and HEK293 cell lines
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View Archive Info| Field | Value | |
| Title |
Antioxidant potential and in vitro cytotoxicity study of Saraca indica extract on lead-induced toxicity in HepG2 and HEK293 cell lines
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| Creator |
Shivhare, Brijesh
Pandey, Maneesha Kumar, Ramesh |
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| Subject |
Antioxidant activity
Cytotoxicity HepG2 and HEK293 cell line MTT assay Saraca indica |
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| Description |
67-74
Saraca indica is an important medicinal plant and the compounds present in this plant are very much helpful in preventing various diseases. Therefore, the aim of the present study was to investigate the antioxidant activity and in vitro investigation of S. indica extract on lead-induced toxicity in HepG2 and HEK293 cell lines. The antioxidant assay was performed by two methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) (ABTS) radical cation scavenging assay. MTT assay was used for in vitro cytotoxic activity of human hepatocellular liver cell (HepG2) and human embryonic kidney (HEK293) cell lines. IC50 values of the DPPH radical scavenging assay for aqueous and ethanolic extracts were 380 and 350 μg/mL, respectively. IC50 values of ABTS+ radical cation scavenging assay for aqueous and ethanolic extracts were 200 and 350 μg/mL, respectively. Cell viability for HepG2 and HEK293 cell lines was found to be 50% at 800 μg/mL concentration for aqueous extract and 1000 μg/mL for ethanolic extract. Data obtained from the MTT assay indicated that S. indica extract significantly increased the viability of the HepG2 and HEK293 cell lines in a dose-dependent manner. Both the aqueous and ethanolic extracts of S. indica are involved in the protection against lead-induced toxicity. Therefore, S. indica extract may be used as a salvage therapy for lead-induced toxicity. Further studies are required to isolate the bioactive compounds from plants for advanced investigation. |
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| Date |
2023-05-11T10:06:10Z
2023-05-11T10:06:10Z 2023-05 |
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| Type |
Article
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| Identifier |
0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/61900 https://doi.org/10.56042/ijnpr.v14i1.1139 |
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| Language |
en
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| Relation |
A61K 36/00, A61K 36/48, A61P 39/00
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| Publisher |
NIScPR-CSIR,India
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| Source |
IJNPR Vol.13(4) [March 2023]
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