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Steroidogenic acute regulatory protein (StAR) gene expression construct: Development, nanodelivery and effect on reproduction in air-breathing catfish, Clarias batrachus

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Title Steroidogenic acute regulatory protein (StAR) gene expression construct: Development, nanodelivery and effect on reproduction in air-breathing catfish, Clarias batrachus
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Creator Rathor PK, Rather MA, Gireesh-Babu P, Kumar K, Purayil SBP, Sharma R*
 
Subject StAR gene Expression vector DNA construct Chitosan nanoparticles Nanodelivery
 
Description Not Available
Steroidogenic acute regulatory protein (StAR) is responsible for the relocation of cholesterol across mito- chondrial membrane in vertebrates and is, therefore, a key factor in regulating the rate and timing of steroidogenesis. In the present study, we developed chitosan nanoparticle (CNP) conjugated StAR gene construct (CNP-pcDNA4-StAR) in a eukaryotic expression vector, pcDNA4/HisMax A. CNPs of 135.4 nm
diameter, 26.7 mV zeta potential and 0.381 polydispersity index were used for conjugation. The loading efficiency (LE) of pcDNA4-StAR construct with CNPs was found to be 86%. After the 24 h of intramuscular injection, the CNP-pcDNA4-StAR plasmid could be detected from testis, brain, kidney and muscle tissues of Clarias batrachus. The transcript levels of important reproductive genes viz. cyp11a1, cyp17a1, 3 -hsd, 17 -hsd and cyp19a1 in CNP-pcDNA4-StAR treated group were initially low up to 24 h, but significantly increased subsequently up to 120 h. In naked pcDNA4-StAR treated group, the mRNA level of 3 -hsd, 17 -hsd and cyp19a1 increased initially up to 24 h, while cyp11a1 and cyp17a1 increased up to 48 h and then started declining. Similar results were obtained for 11-Ketotestosterone and 17 -estradiol. The results indicate relatively long lasting effects of nano-conjugated construct compared to the construct alone. Furthermore, the histopathology of gonads and liver authenticates its possible role in the gonadal development in fish without any adverse effect.
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Date 2023-05-12T03:59:10Z
2023-05-12T03:59:10Z
2017-06-21
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/77122
 
Language English
 
Relation Not Available;
 
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