Steroidogenic acute regulatory protein (StAR) gene expression construct: Development, nanodelivery and effect on reproduction in air-breathing catfish, Clarias batrachus
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Title |
Steroidogenic acute regulatory protein (StAR) gene expression construct: Development, nanodelivery and effect on reproduction in air-breathing catfish, Clarias batrachus
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Creator |
Rathor PK, Rather MA, Gireesh-Babu P, Kumar K, Purayil SBP, Sharma R*
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Subject |
StAR gene Expression vector DNA construct Chitosan nanoparticles Nanodelivery
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Description |
Not Available
Steroidogenic acute regulatory protein (StAR) is responsible for the relocation of cholesterol across mito- chondrial membrane in vertebrates and is, therefore, a key factor in regulating the rate and timing of steroidogenesis. In the present study, we developed chitosan nanoparticle (CNP) conjugated StAR gene construct (CNP-pcDNA4-StAR) in a eukaryotic expression vector, pcDNA4/HisMax A. CNPs of 135.4 nm diameter, 26.7 mV zeta potential and 0.381 polydispersity index were used for conjugation. The loading efficiency (LE) of pcDNA4-StAR construct with CNPs was found to be 86%. After the 24 h of intramuscular injection, the CNP-pcDNA4-StAR plasmid could be detected from testis, brain, kidney and muscle tissues of Clarias batrachus. The transcript levels of important reproductive genes viz. cyp11a1, cyp17a1, 3 -hsd, 17 -hsd and cyp19a1 in CNP-pcDNA4-StAR treated group were initially low up to 24 h, but significantly increased subsequently up to 120 h. In naked pcDNA4-StAR treated group, the mRNA level of 3 -hsd, 17 -hsd and cyp19a1 increased initially up to 24 h, while cyp11a1 and cyp17a1 increased up to 48 h and then started declining. Similar results were obtained for 11-Ketotestosterone and 17 -estradiol. The results indicate relatively long lasting effects of nano-conjugated construct compared to the construct alone. Furthermore, the histopathology of gonads and liver authenticates its possible role in the gonadal development in fish without any adverse effect. Not Available |
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Date |
2023-05-12T03:59:10Z
2023-05-12T03:59:10Z 2017-06-21 |
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Type |
Research Paper
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Identifier |
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Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77122 |
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Language |
English
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Relation |
Not Available;
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