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Identification, ontogeny and expression analysis of a novel laboratory of genetics and physiology 2 (LGP2) transcripts in Asian seabass, Lates calcarifer.

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Title Identification, ontogeny and expression analysis of a novel laboratory of genetics and physiology 2 (LGP2) transcripts in Asian seabass, Lates calcarifer.
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Creator Paria, A., Deepika, A., Sreedharan, K., Makesh, M., Chaudhari, A., Purushothaman, C.S., Rajendran, K.V*.,
 
Subject Laboratory of genetics and physiology 2 LGP2 RLR PRR Asian seabass Lates calcarifer Bacterial infection Poly I:C Gene expression Ontogeny
 
Description Not Available
LGP2 (laboratory of genetics and physiology 2) is an important member of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), which plays a significant role in antiviral innate immunity. In this study, we have cloned the full-length cDNA sequence of LGP2 from Asian seabass, Lates calcarifer (AsLGP2). The complete AsLGP2 cDNA sequence consisted of 2586 nucleotides encoding a putative protein of 681 amino acids with a molecular mass of 77.6 kDa. From the AsLGP2 protein, four different conserved
domains were predicted: a DExDc (DEAD/DEAH box helicase domain), a bacterial type III restriction enzyme domain (RES III), a HELICc (Helicase superfamily c-terminal domain) and a RIG-I_C-RD (RIG-I C- terminal regulatory domain). The transcript of AsLGP2 could be detected in all the 11 tissues tested in healthy animals with high expression noticed in tissues facing external environment such as gill, hindgut
and skin. The ontogenic expression profile of AsLGP2 implies a possible maternal transfer of this gene as it has been detected in all early embryonic developmental stages along with unfertilized eggs. Viral analogue, poly I:C, injection resulted in rapid up-regulated expression in different tissues with the highest modulation of expression observed in kidney followed by liver and gill. A rapid response of AsLGP2 expression was also observed in the different tissues of Vibrio alginolyticus-injected L. calcarifer,
while significant change in expression was noticed following Staphylococcus aureus infection. Similarly, exposure to different pathogen-mimicking microbial analogues such as poly I:C, LPS and PGN resulted in enhanced expression of AsLGP2 in SISK cell-line. Taking together, these observations suggest that AsLGP2 can act as both antiviral and antibacterial cytosolic receptor and may play a significant role in embryonic and larval development in marine euryhaline teleosts like Asian seabass.
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Date 2023-05-12T03:57:31Z
2023-05-12T03:57:31Z
2017-01-21
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/77117
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER