Generation of acid resistant virus like particles of vaccine strains of foot-and mouth disease virus (FMDV)
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Title |
Generation of acid resistant virus like particles of vaccine strains of foot-and mouth disease virus (FMDV)
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Creator |
P.R. Deepak
P. Saravanana J.K. Biswal S.H. Basagoudanavar H.J. Dechamma V. Umapathi B.P. Sreenivasa R.P. Tamilselvan N. Krishnaswamy I. Zaffer A. Sanyal |
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Subject |
Foot-and-mouth disease virus
Virus like particles Acid resistant VLPs Vaccine viruses Antigenicity TEM Insect cell expression system Sucrose density gradient LPBE Sandwich ELISA |
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Description |
Not Available
Foot-and-mouth disease (FMD) is a contagious viral disease affecting cloven hoofed livestock. Insect cell expressed virus like particles (VLPs) are potential alternative to overcome the limitations of inactivated vaccine. However, at pH < 6.5, virus particles disassociate into pentameric structure resulting in loss of antigenicity. Accordingly, we generated seven mutant VLPs containing mutations in the structural genes of FMDV vaccine strains (N17D and/or H145Y for serotypes O/IND/R2/75 and Asia1/IND/63/72; and H142D for serotype A/IND/40/00) by PCR based site directed mutagenesis. Acid resistant VLPs produced by baculovirus expression system were tested for acid stability at pH 7.5, 6.5, 6.0 and 5.5 followed by reactivity in sandwich-ELISA (s-ELISA), which revealed mutant-1 (N17D) of serotype O and Asia1 retained the antigenicity in s-ELISA even at pH 5.5 as compared to other VLPs and wild-types. Further, the 75S empty capsids obtained in sucrose density gradient, when tested in liquid phase blocking ELISA (LPBE) in comparison to cell culture antigen indicated that the VLPs were stable at acidic pH. Transmission electron microscopy of OM-1 confirmed the intact morphology of the empty VLPs. It is concluded that acid resistant VLPs could be useful for developing new generation vaccine or diagnostic for FMDV. Not Available |
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Date |
2023-05-12T03:23:29Z
2023-05-12T03:23:29Z 2019-07-01 |
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Type |
Research Paper
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Identifier |
Deepak PR, Saravanan P, Biswal JK, Basagoudanavar SH, Dechamma HJ, Umapathi V, Sreenivasa BP, Tamilselvan RP, Krishnaswamy N, Zaffer I, Sanyal A. Generation of acid resistant virus like particles of vaccine strains of foot-and-mouth disease virus (FMDV). Biologicals. 2019 Jul;60:28-35. doi: 10.1016/j.biologicals.2019.06.003. Epub 2019 Jun 17. Erratum in: Biologicals. 2020 Jan;63:106. PMID: 31221554.
1045-1056 http://krishi.icar.gov.in/jspui/handle/123456789/77084 |
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Language |
English
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Relation |
Not Available;
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ELSEVIER
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