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Expression of bovine interleukin 15 in Pichia pastoris and study on its biological activity: a T-cell activator

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Title Expression of bovine interleukin 15 in Pichia pastoris and study on its biological activity: a T-cell activator
Not Available
 
Creator N. Vijay
J. Lijo
S. Barathiraja
P. A. V. Gangadhara
H. J. Dechamma
G. R. Reddy
 
Subject Bovine Interleukin-15 (bIL-15)
Carnitine Palmitoyl Transferase 1a (cpt1a)
Memory CD8 + T Cells
Pichia Pastoris
Bcl2
 
Description Not Available
Interleukin 15 (IL-15) plays crucial role as stimulatory cytokine in generation and proliferation of CD8+ T memory cells. The present study was initiated to analyze the role of yeast-expressed bovine IL-15 in inducing the CD8+ T memory cells. The bIL-15 was cloned in pPICZαA expression vector. The expressed bovine IL-15 was purified by anion exchange chromatography and further characterized using SDS-PAGE and Western blotting. Biological activity of the purified protein was evaluated in vitro through induction of Bcl2 in IL-15 stimulated PBMCs was measured using qPCR and the phosphorylation of STAT3 was confirmed by immuno-staining of HEK273 cells. From the recent research studies, it was evident that the fatty acid oxidation catalyzed by Carnitine Palmitoyl Transferase 1a (Cpt1a) is a critical step during the conversion of effector CD8+ T cells to memory CD8+ T cells which is induced by IL-15. There were no research studies revealed the role of IL-15 on activating memory CD8+ T cells by influencing the Cpt1a in Bovines was documented; yet and in the present study, we evaluated that bovine IL-15 could induce the expression of Cpt1a in IL-15 treated bovine PBMCs and helps in memory cell induction.
Not Available
 
Date 2023-05-12T03:22:52Z
2023-05-12T03:22:52Z
2019-04-15
 
Type Research Paper
 
Identifier Vijay N, Lijo J, Barathiraja S, Gangadhara PAV, Dechamma HJ, Reddy GR. Expression of bovine interleukin 15 in Pichia pastoris and study on its biological activity: a T-cell activator. Anim Biotechnol. 2020 Aug;31(4):357-364. doi: 10.1080/10495398.2019.1604381. Epub 2019 Apr 15. PMID: 30987516.
1049-5398
http://krishi.icar.gov.in/jspui/handle/123456789/77083
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis