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Expression and purification of biologically active bovine Interferon λ3 (IL28B) in Pichia pastoris: Protein Expression and Purification

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Title Expression and purification of biologically active bovine Interferon λ3 (IL28B) in Pichia pastoris: Protein Expression and Purification
Not Available
 
Creator S. Barathiraja
P.A.V. Gangadhara
V. Umapathi
H.J. Dechamma
G.R. Reddy
 
Subject Interferon λ3
Expression in Pichia pastoris
SP-Sepharose chromatography
Interferon stimulated genes (ISGs)
 
Description Not Available
Interferon lambda-3 (IFNλ3) which is also known as IL28B is a member of type III Interferons which are structurally and genetically different from type I Interferons. These Interferons induce signal transduction pathways similar to type I Interferons which results in the activation of Interferon Stimulated Genes (ISGs). This group of Interferons are tissue specific and reported to have antiviral activity. In the present communication, we report the expression of bovine IFNλ3 gene (coding for the mature protein) in Pichia pastoris, purification of the expressed protein and evaluation of its biological activity. About 19 kDa protein expressed by the transformed Pichia cells, secreted into the media and the protein was purified by SP-Sepharose ion exchange chromatography with NaCl stepwise gradient elution. Specificity of the protein was confirmed by Western blotting. Pichia expressed IFNλ3 was found to be biologically active, as it induced ISGs (Mx protein, OAS and PKR genes) in bovine PBMCs. Further it was also found to modulate Th1/Th2 cytokines expression in the stimulated bovine PBMCs.
Not Available
 
Date 2023-05-12T03:16:11Z
2023-05-12T03:16:11Z
2018-05-01
 
Type Research Paper
 
Identifier Barathiraja S, Gangadhara PAV, Umapathi V, Dechamma HJ, Reddy GR. Expression and purification of biologically active bovine Interferon λ3 (IL28B) in Pichia pastoris. Protein Expr Purif. 2018 May;145:14-18. doi: 10.1016/j.pep.2017.12.007. Epub 2017 Dec 24. PMID: 29278743.
1046-5928
http://krishi.icar.gov.in/jspui/handle/123456789/77078
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER