Record Details

Novel immunogenic baculovirus expressed virus-like particles of foot-and-mouth disease (FMD) virus protect guinea pigs against challenge.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Novel immunogenic baculovirus expressed virus-like particles of foot-and-mouth disease (FMD) virus protect guinea pigs against challenge.
Not Available
 
Creator S A Bhat
P Saravanan
M Hosamani
S H Basagoudanavar
B P Sreenivasa
R P Tamilselvan
R Venkataramanan
 
Subject Vaccine
foot-and-mouth disease
virus antigens
 
Description Not Available
Vaccination is a well accepted strategy for control of foot-and-mouth disease (FMD) in endemic countries. Currently, chemically inactivated virus antigens are used for preparation of FMD vaccine. To develop a non-infectious and safe recombinant vaccine, we expressed structural polypeptide of FMDV (O/IND/R2/75) using baculovirus expression system. We show that inclusion of mutated viral 3C protease in frame with the polypeptide (P1-2A), enhanced the yield of structural proteins. The structural proteins retained antigenicity and assembled into empty virus-like particles (VLPs). Immunization of guinea pigs with purified fractions of the VLPs resulted in humoral and cell mediated immune response by 4 weeks. The VLPs elicited comparable humoral immune response and relatively higher cell mediated immune response, when compared to conventional vaccine in guinea pigs. Further, up to 70% of the VLP immunized guinea pigs were protected against challenge with homologous guinea pig adapted virus. Our results highlight the application of recombinant FMDV VLPs in FMD vaccination.
Not Available
 
Date 2023-05-12T03:17:13Z
2023-05-12T03:17:13Z
2013-12-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77079
 
Language English
 
Relation Not Available;
 
Publisher Research in Veterinary Science