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Immunoreactivity and trypsin sensitivity of insect cell expressed virus like particles of serotype Asia1 FMD virus.

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Title Immunoreactivity and trypsin sensitivity of insect cell expressed virus like particles of serotype Asia1 FMD virus.
Not Available
 
Creator Suresh H Basagoudanavar
Madhusudan Hosamani
R P Tamil Selvan
Paramasivam Saravanan
B P Sreenivasa
Ramamurthy Venkataramanan
 
Subject Foot-and-mouth disease
virus-like particles
serotype Asia-1
 
Description Not Available
Foot-and-mouth disease (FMD) is an important infection affecting the health and productivity of cloven-hoofed livestock. Development of improved vaccines and diagnostic reagents is being explored to facilitate the disease control. There is an emerging interest in virus-like particles (VLPs), as their constituent structural proteins are the major immunogens. The VLPs are similar to natural virus particles but lack viral nucleic acid. The objective of the present study was to express the VLPs of FMD virus (FMDV) serotype Asia-1 (IND 63/72), using baculovirus system and characterize them for antigenic structure. The VLPs expressed in insect cells showed immunoreactivity similar to inactivated cell culture FMDV. Further they possess similar sensitivity to trypsin as the inactivated cell culture FMDV, suggesting that trypsin-sensitive antigenic sites could be similarly arranged. Our findings suggest that the FMD VLPs have similar antigenic conformational feature like the wild type virus, thus supporting their utility in development of non-infectious FMD vaccines and/or diagnostic assays.
Not Available
 
Date 2023-05-12T03:22:15Z
2023-05-12T03:22:15Z
2015-03-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77082
 
Language English
 
Relation Not Available;
 
Publisher Acta virologica