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Detection of replication competent adenovirus upon serial passaging of recombinant adenovirus expressing FMDV capsid proteins.

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Title Detection of replication competent adenovirus upon serial passaging of recombinant adenovirus expressing FMDV capsid proteins.
Not Available
 
Creator R Kumar
H B Suresh
B P Sreenivasa
 
Subject HEK-293
Human adenovirus type 5
FMD virus
 
Description Not Available
Replication deficient human adenovirus type 5 (hAd5) is an important gene delivery vehicle and has been used in various fields of biomedical sciences such as gene therapy, cancer therapy and vaccination. Inspite of its various useful features, emergence of replication competent adenovirus (RCA) in recombinant virus stocks is a great concern. In the present study, recombinant adenovirus expressing foot-and-mouth disease virus serotype-O capsid proteins was propagated in HEK-293 cells and purified by CsCl density gradient ultracentrifugation. The virus was serially passaged in HEK-293 cells and at passage level (P) 2–4, 6, 8 and 12, tested for the presence of RCA. A vector dose of 2 × 108 and 1 × 1010 TCID50 of the virus was used in cell line bioassay and PCR, respectively. Our results demonstrated that the PCR is more sensitive and rapid technique for the detection of RCA in recombinant adenovirus stocks as early as at P3, whereas the bioassay detected the RCA at P8.
Not Available
 
Date 2023-05-12T03:58:00Z
2023-05-12T03:58:00Z
2015-05-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77120
 
Language English
 
Relation Not Available;
 
Publisher Biologicals