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Eri silkworm (Samia ricini), a non- mulberry host system for AcMNPV mediated expression of recombinant proteins.

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Title Eri silkworm (Samia ricini), a non- mulberry host system for AcMNPV mediated expression of recombinant proteins.
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Creator Madhusudan Hosamani
Suresh H. Basagoudanava
B P Sreenivasa
Shigeki Inumaru
Chandish R Ballal
Ramamurthy Venkataramanan
 
Subject baculovirus
Eri silkworm
foot-and-mouth disease virus
 
Description Not Available
The baculovirus expression system (BVES) based on Autographa californica nucleopolyhedrovirus (AcMNPV) is widely used for the expression of eukaryotic proteins. Several insect cells/larvae that are permissive to AcMNPV have been routinely used as hosts to express heterologous proteins. Domesticated Eri silkworm (Samia ricini), reared in many parts of India, Japan and China, is a non-mulberry silkworm. The present study shows that the Eri silkworm larvae are susceptible to intra-haemocoelical inoculation of AcMNPV. The virus replicates in the larva, as indicated by an increased viral loads in the haemolymph upon injection of a recombinant AcMNPV carrying green fluorescent protein gene. The virus showed localized replication in different tissues including the fat body, haemocytes, tracheal matrix and in the Malphigian tubules. The larval system was successfully used to express heterologous protein, by infecting with a recombinant AcMNPV carrying the 3ABC coding sequence of foot-and-mouth disease virus (FMDV). The study shows that the Eri silkworm larva can be a potential alternative bioreactor, for scaling up of the recombinant proteins employing the baculovirus system.
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Date 2023-05-12T03:52:48Z
2023-05-12T03:52:48Z
2015-12-20
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77105
 
Language English
 
Relation Not Available;
 
Publisher Journal of Biotechnology