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DNA prime-protein boost strategy with replicase-based DNA vaccine against foot-and-mouth disease in bovine calves

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Title DNA prime-protein boost strategy with replicase-based DNA vaccine against foot-and-mouth disease in bovine calves
Not Available
 
Creator Pervaiz A. Dar
Veluvarthy S. Suryanaryana
G. Nagarajan
Golla R. Reddy
Hosur J. Dechamma
Ganesh Kondabattula
 
Subject Foot-and-mouth disease
Replicase-based DNA vaccine
Multivalent epitope vaccine
Prime-boost vaccination regime
Immune response
Cattle
 
Description Not Available
The limited efficacy of DNA vaccines against foot-and-mouth disease (FMD) in cattle and other natural hosts has prompted a search for a more effective vaccination regimen. In this study we tested a DNA prime-protein boost vaccination strategy against FMD in bovine calves. We used purified recombinant FMDV specific multi-epitope protein (rMEG990) and an optimized sindbis virus replicase-based DNA vaccine expressing this protein (pSinCMV-Vac-MEG990). We demonstrate that vaccination with a low dose of pSinCMV-Vac-MEG990 (10 μg/animal) and subsequently boosting with rMEG990 resulted in induction of neutralizing antibodies, IFN-γ production and protection against homologous virus challenge. However, vaccination with a high dose of pSinCMV-Vac-MEG990 (100 μg/animal) and boosting with rMEG990 resulted in significantly lower immune responses and more severity to the challenge test. Additionally, we show that the post-vaccinal IFN-γ levels in animals correlated positively to their protection against FMDV challenge. These findings suggest that a replicase-based DNA vaccine in proper prime-boost combination may offer an efficient vaccine strategy against FMDV and that IFN-γ could be used as an additional immune parameter to predict protection against FMDV infection.
Not Available
 
Date 2023-05-12T04:29:01Z
2023-05-12T04:29:01Z
2013-04-12
 
Type Research Paper
 
Identifier Dar PA, Suryanaryana VS, Nagarajan G, Reddy GR, Dechamma HJ, Kondabattula G. DNA prime-protein boost strategy with replicase-based DNA vaccine against foot-and-mouth disease in bovine calves. Vet Microbiol. 2013 Apr 12;163(1-2):62-70. doi: 10.1016/j.vetmic.2012.12.017. Epub 2012 Dec 25. PMID: 23305616.
0378-1135
http://krishi.icar.gov.in/jspui/handle/123456789/77167
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER