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Baculovirus expression and purification of peste- des-petits-ruminants virus nucleocapsid protein and its application in diagnostic assay

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Title Baculovirus expression and purification of peste- des-petits-ruminants virus nucleocapsid protein and its application in diagnostic assay
Not Available
 
Creator Suresh H Basagoudanavar
Madhusudan Hosamani
D Muthuchelvan
R P Singh
R Santhamani
B P Sreenivasa
P Saravanan
A B Pandey
R K Singh
Ramamurthy Venkataramanan
 
Subject PPR virus
Nucleocapsid N protein
ELISA
 
Description Not Available
Peste-des-petits-ruminants (PPR) is a contagious and highly devastating disease of small ruminants. For control of endemic PPR, adequate supply of affordable and reliable diagnostics is critical for effective surveillance, along with the use of highly efficacious live vaccines that are currently available. The nucleocapsid (N) protein of PPR virus (PPRV) is an important candidate antigen for developing specific diagnostic, as it is a major viral protein being highly immunogenic and conserved among the structural proteins. In the present study, we expressed the N protein of PPRV (Sungri/96 strain), in baculovirus expression system and purified using affinity column chromatography. The recombinant protein reacted well with PPRV anti-N monoclonal antibodies and PPRV-specific polyclonal antiserum, suggesting that the expressed protein was authentic and in native form. The recombinant protein was evaluated as antigen in the diagnostic ELISA as reference positive control in place of whole virus antigen. The utility of recombinant PPRV N protein circumvents the need to use live PPRV antigen in the routinely used diagnostics targeting â€~N’ protein of PPRV, thus allowing large-scale field application of the test.
Not Available
2023-05-12T04:08:52Z
2023-05-12T04:08:52Z
2018-09-01
Article
Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77156
English
Not Available;
Biologicals

oai:krishi.icar.gov.in:123456789/778632023-05-30T04:05:41Zcom_123456789_62com_123456789_4col_123456789_195
Adoption level and constraints in vegetable production technology
Not Available
R S Suman
Adoption level, constraints, vegetable production technology
Not Available
The yield level of vegetable which is comparatively low at present need to be increased substantianly. Higher vegetable production can be achieved by adoption of all the recommended technologies by large number of farmers. Adoption of vegetable production technology was studied during 2004-09 at Kullu district of Himachal Pradesh. Majority of the farmers showed low level of overall adoption of recommended technology. Weedicide application in nursery, weedicide application in main field, plant population, seed treatment with fungicides, organic manure application and nursery area were not adopted by the majority of the farmers. Non ovailability of high-yielding varieties, High cost of labour, Lack of awareness of new technology and weak extension activities at the village level were the major constraints faced by the farmers. Therefore, it was necessary to intensify the extension efforts to increase their knowledge level and adoption of recommended vegetable technologies, which would help in increasing the yield, of vegetable at farm level.
Not Available
2023-05-30T04:05:41Z
2023-05-30T04:05:41Z
2011-01-01
Research Paper
Suman, R. S. (2011). Adoption level and constraints in vegetable production technology. International Journal of Agricultural Sciences, 7 (1): 227-230
0973-130X
http://krishi.icar.gov.in/jspui/handle/123456789/77863
English
Not Available;
Hind Agri-Horti Society

oai:krishi.icar.gov.in:123456789/771732023-05-12T04:40:14Zcom_123456789_62com_123456789_4col_123456789_195
Efficient inhibition of foot-and-mouth disease virus replication in vitro by artificial microRNA targeting 3D polymerase
Not Available
S H Basagoudanavar
H B Ranjitha
M Hosamani
M Kolangath Sujit
R P Tamil Selvan
B P Sreenivasa
P Saravanan
A Sanyal
R Venkataramanan
foot-and-mouth disease
RNA interference
virus inhibition.
Not Available
Foot-and-mouth disease (FMD) is a devastating acute viral disease of livestock with cloven hooves. Among various therapeutic control measures, RNA interference (RNAi) is one of the methods being explored to inhibit FMD virus replication and spread. The RNAi is achieved by short hairpin RNAs or artificial microRNAs (amiRNAs). Utility of amiRNAs as antiviral, targeting conserved regions of the viral genome is gaining importance. However, delivery of miRNA in vivo is still a challenge. In this study, the efficacy of amiRNAs in preventing FMD virus replication in a permissive cell culture system was investigated, by generating stable cell lines expressing amiRNAs targeting three functional regions of the FMD virus (FMDV) genome (IRES, 3B3 and 3D). The results showed that amiRNA targeting 3D polymerase is relatively more efficient. However, expression of multiple microRNAs targeting the three regions did not exhibit additive effect. The data suggest that 3D specific miRNA is a potential valid strategy in developing novel antiviral measures against FMDV infection.
Not Available
2023-05-12T04:40:14Z
2023-05-12T04:40:14Z
2019-01-01
Article
Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77173
English
Not Available;
Acta virologica

oai:krishi.icar.gov.in:123456789/771712023-05-12T04:33:37Zcom_123456789_62com_123456789_4col_123456789_195
Development of scale to measure attitude of animal husbandry personnel towards using ICAR-IVRI Crystoscope
Not Available
SP Sunil Kumar, BP Singh, Mahesh Chander, Ram Singh Suman
Crystoscope, Attitude scale,Animal husbandry personnel
Not Available
The attitude of animal husbandry personnel toward any animal husbandry technology isan important factor in evaluating the technology and will also aid in the development offuture innovative technologies. Based on Likert’s summative rating technique of scaledevelopment, a scale was constructed in the year 2020 to measure the attitude of animalhusbandry personnel toward using the ICAR-IVRI Crystoscope. The scale consisted offinal 16 statements, including 8 positive and 8 negative statements. Reliability of the scalewas calculated using Cronbach’s alpha method which was found to be 0.824 and reflectsthe high reliability of the measuring instrument. The validity of the scale was tested byexpert’s judgment. The reliability and validity of the scale indicate its consistency andprecision of the results. This scale can be used to measure the attitude of animal husbandrypersonnel in similar situation beyond the study area with suitable modifications.
Not Available
 
Date 2023-05-12T04:33:37Z
2023-05-12T04:33:37Z
2021-10-01
 
Type Research Paper
 
Identifier 38. SP Sunil Kumar, BP Singh, Mahesh Chander, Ram Singh Suman (2021). Development of scale to measure attitude of animal husbandry personnel towards using ICAR-IVRI Crystoscope. Indian Journal of Extension Education, 57 (4): 150-152
ISSN 0537-1996 (Print), ISSN 2454-552X (Online)
http://krishi.icar.gov.in/jspui/handle/123456789/77171
 
Language English
 
Relation Not Available;
 
Publisher Indian Society of Extension Education