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Evaluation of Different Methods for Conversion of Whole Virion Particle (146S) of FMDV into 12S Subunits and Application in Characterization of Monoclonal Antibodies

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Title Evaluation of Different Methods for Conversion of Whole Virion Particle (146S) of FMDV into 12S Subunits and Application in Characterization of Monoclonal Antibodies
Not Available
 
Creator G S Krishna
S Shanmuganathan
R Manikandan
M Hosamani
V Bhanuprakash
R. P. Tamil Selvan
S H Basagoudanavar
A Sanyal
B P Sreenivasa
 
Subject Foot-and-mouth disease virus
Monoclonal antibodies
Whole virion particle
 
Description Not Available
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven hoofed animals
caused by FMD virus classified under genus Apthovirus and Picornaviridae family.
Monoclonal antibodies (mAb) to FMDV provides best tool for the development of reliable
diagnostics and there is a need for the development of mAbs that specifically recognize
either 146S or 12S for the quality control analysis of FMDV vaccines. In the present study,
we developed mAbs specific to FMDV serotype O and characterized their specificity in
recognition of 146S or 12S particles. Three different methods were evaluated for the
conversion of 146S into 12S subunits that includes heat method, strong acid and mild acid
methods for testing the reactivity of mAbs with these antigens by double antibody
sandwich ELISA. Mild heating of 146S antigens at 560C for 1hour and treatment of 146S
antigen with weak acid (0.5M NaH2PO4, pH 4.5 - 5.5) resulted in complete conversion of
146S into 12S. Where as in strong acid method (1N HCl with pH below 4.5), 146S treated
with 1N HCl resulted in loss of antigenicity of 12S subunits that reflected in absence of
reactivity in ELISA. This may mislead to consider the mAb as 146S specific. This study
revealed that mAb binding epitopes of all monoclonals are commonly shared among 146S
and 12S antigens. Further it helps in understanding the specificity of mAbs to 146S and
12S particles which will be useful for application of mAbs in different diagnostic assays.
Not Available
 
Date 2023-05-12T04:42:40Z
2023-05-12T04:42:40Z
2019-08-10
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77175
 
Language English
 
Relation Not Available;
 
Publisher International Journal of Current Microbiology and Applied Sciences