Expression of vp1 protein of serotype a and o of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs
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Title |
Expression of vp1 protein of serotype a and o of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs
Not Available |
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Creator |
J. P. Rao
P. Agrawal R. Mohammad S. K. Rao G. R. Reddy H. J. Dechamma V. V. S sury¬anarayana |
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Subject |
foot-and-mouth disease
sunnhemp Agrobacterium tumefaciens FMDV-VP1 gene serotype O and A in planta transformation transgenic plants bivalent vaccine |
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Description |
Not Available
Recently, transgenic plants expressing immunogenic proteins of foot-and-mouth disease virus (FMDV) have been used as oral or parenteral vaccines against foot-and-mouth disease (FMD). They exhibit advantages like cost effectiveness, absence of processing, thermostability, and easy oral application. FMDV VP1 protein of single serotype has been mostly used as immunogen. Here we report the development of a bivalent vaccine with tandem-linked VP1 proteins of two serotypes, A and O, present in transgenic forage crop Crotalaria juncea. The expression of the bivalent protein in the transgenic plants was confirmed by Western blot analysis. Guinea pig reacted to orally or parenterally applied vaccine by humoral as well as cell-mediated immune responses including serum antibodies and stimulated lymphocytes, respectively. The vaccine protected the animals against a challenge with the virus of serotype A as well as O. This is the first report on the development of a bivalent FMD vaccine using a forage crop. Not Available |
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Date |
2023-05-12T04:32:45Z
2023-05-12T04:32:45Z 2012-01-01 |
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Type |
Research Paper
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Identifier |
Rao JP, Agrawal P, Mohammad R, Rao SK, Reddy GR, Dechamma HJ, S Suryanarayana VV. Expression of VP1 protein of serotype A and O of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs. Acta Virol. 2012;56(2):91-9. doi: 10.4149/av_2012_02_91. PMID: 22720698.
0001-723X http://krishi.icar.gov.in/jspui/handle/123456789/77170 |
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Language |
English
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Relation |
Not Available;
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Publisher |
PubMed Central
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