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Comparative efficacy of different methods in the generation of 12S particles from 146S particles of FMDV serotype A and their detection using serotype (146S) specific monoclonal antibodies

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Title Comparative efficacy of different methods in the generation of 12S particles from 146S particles of FMDV serotype A and their detection using serotype (146S) specific monoclonal antibodies
Not Available
 
Creator S Shanmuganathan
G S Krishna
S R Nagasupreeta
K Kavya
G Shreya
R P Tamil Selvan
B P Sreenivasa
V Bhanuprakash
 
Subject Viral disease
antibodies
monoclonal
 
Description Not Available
Foot-and-mouth disease [FMD] is a highly infectious and contagious viral disease
of domestic and wild cloven hoofed animals. The disease is controlled by
vaccination using inactivated vaccine as one of the important options. The
integrity of 146S plays an important role in the efficacy of the vaccine. The
currently applied methods like density gradient to check the integrity of the 146S
particles are besotted with certain disadvantages. Therefore, we generated
monoclonal antibodies (mAbs) specific to 146S of FMDV serotype A [Indian
strain]. To test these mAbs for their specificity to 146S, conversion of 146S into
12S is a must. Normally, three methods like strong [1N HCl] and weak acid
[Na2HPO4] methods; and heat method are followed in the conversion of 146S into
12S. Upon comparison of these methods in the present study, consistent results
were obtained using heat method at 560C and 600C each for half an hour and one
hour. However, the former two methods were very inconsistent in yielding 12S
from 146S particle due to slight variation in the pH. Hence, we optimized heat
method for efficient conversion of 146S particle to 12S particle of FMDV
serotype A [A/INDIA/40/00], an Indian vaccine strain. The results are ascertained
applying serotype specific [146S] monoclonal antibody based double antibody
sandwich ELISA. However, the method needs to be evaluated using more number
of 146S samples.
Not Available
 
Date 2023-05-12T04:26:32Z
2023-05-12T04:26:32Z
2020-02-10
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77164
 
Language English
 
Relation Not Available;
 
Publisher International Journal of Current Microbiology and Applied Sciences