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Expression of bovine (Bos indicus) interleukin-18 in Escherichia coli and its biological activity

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Title Expression of bovine (Bos indicus) interleukin-18 in Escherichia coli and its biological activity
Not Available
 
Creator K Siva Reddy
Muralidhar Rao
Dowlathabad
Dechamma H. J
Suryanarayana V. V.S.
G.R. Reddy
 
Subject Bovine
interlukin-18
E. coli
 
Description Not Available
IL-18 modulates immune functions by inducing IFN-γ production and promoting Th1 immune responses. In the present study, we amplified and cloned the sequence (582 bp) encoding full-length bovine IL-18 from PBMC stimulated with PHA. The nucleotide and the deduced amino acid sequence of Bos indicus IL-18 showed an identity of 86-98% compared with IL-18 sequences of other ruminants. The insert was subcloned into a pET 32a vector and expressed in Escherichia coli as a fusion protein and the matured protein was obtained by caspase I treatment. The specificity of these proteins was confirmed by western blotting. The biological activity of the purified protein was analyzed by its ability to induce IFN-γ production in PBMC measured by ELISA and qPCR.
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Date 2023-05-12T04:55:52Z
2023-05-12T04:55:52Z
2010-08-27
 
Type Research Paper
 
Identifier Reddy KS, Rao DM, Dechamma HJ, Suryanarayana VV, Reddy GR. Expression of bovine (Bos indicus) interleukin-18 in Escherichia coli and its biological activity. Microbiol Immunol. 2010 Sep;54(9):564-7. doi: 10.1111/j.1348-0421.2010.00241.x. PMID: 20840156.
PMID: 20840156
http://krishi.icar.gov.in/jspui/handle/123456789/77179
 
Language English
 
Relation Not Available;
 
Publisher Wiley Online Library