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The plasmid constructs producing shRNA corresponding to the conserved 3D polymerase of Foot and Mouth Disease virus protects guinea pigs against challenge virus

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Title The plasmid constructs producing shRNA corresponding to the conserved 3D polymerase of Foot and Mouth Disease virus protects guinea pigs against challenge virus
Not Available
 
Creator Dechamma Hosur Joyappa
Sarika Sasi
Kumar C. Ashok
Golla Rama Reddy
Veluvarthy V. S. Suryanarayana
 
Subject Foot-and-mouth disease virus
ShRNA
RNAi
 
Description Not Available
RNA interference (RNAi) has been used as an effective antiviral strategy for its specific silencing of viral gene expression in mammalian cells. In this study, shRNA targeting two regions of Foot and Mouth Disease Virus (FMDV) i.e. 3D and 5′UTR which are very essential in virus replication were evaluated. The constructs were made using h7K RNA polymerase III promoter. We investigated in vivo inhibitory effect of shRNA on FMDV replication in BHK-21 cells and guinea pigs. The results showed that transfection of 3D shRNA could reduce virus growth by three folds when cells were challenged with 102 TCID50 of FMDV. Pretreated guinea pigs with 3DshRNA were protected 80% with 103 GPID50 of FMDV. As a first report in guinea pigs which are recognized animal model for FMD vaccine potency testing, the study suggests that shRNA could be a viable therapeutic approach to control severity of FMD infection and spread.
Not Available
 
Date 2023-05-12T04:57:57Z
2023-05-12T04:57:57Z
2008-09-23
 
Type Research Paper
 
Identifier Joyappa DH, Sasi S, Ashok KC, Reddy GR, Suryanarayana VV. The plasmid constructs producing shRNA corresponding to the conserved 3D polymerase of Foot and Mouth Disease virus protects guinea pigs against challenge virus. Vet Res Commun. 2009 Mar;33(3):263-71. doi: 10.1007/s11259-008-9174-3. Epub 2008 Sep 23. PMID: 18810649.
1573-7446
http://krishi.icar.gov.in/jspui/handle/123456789/77182
 
Language English
 
Relation Not Available;
 
Publisher Springer