The plasmid constructs producing shRNA corresponding to the conserved 3D polymerase of Foot and Mouth Disease virus protects guinea pigs against challenge virus
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Title |
The plasmid constructs producing shRNA corresponding to the conserved 3D polymerase of Foot and Mouth Disease virus protects guinea pigs against challenge virus
Not Available |
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Creator |
Dechamma Hosur Joyappa
Sarika Sasi Kumar C. Ashok Golla Rama Reddy Veluvarthy V. S. Suryanarayana |
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Subject |
Foot-and-mouth disease virus
ShRNA RNAi |
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Description |
Not Available
RNA interference (RNAi) has been used as an effective antiviral strategy for its specific silencing of viral gene expression in mammalian cells. In this study, shRNA targeting two regions of Foot and Mouth Disease Virus (FMDV) i.e. 3D and 5′UTR which are very essential in virus replication were evaluated. The constructs were made using h7K RNA polymerase III promoter. We investigated in vivo inhibitory effect of shRNA on FMDV replication in BHK-21 cells and guinea pigs. The results showed that transfection of 3D shRNA could reduce virus growth by three folds when cells were challenged with 102 TCID50 of FMDV. Pretreated guinea pigs with 3DshRNA were protected 80% with 103 GPID50 of FMDV. As a first report in guinea pigs which are recognized animal model for FMD vaccine potency testing, the study suggests that shRNA could be a viable therapeutic approach to control severity of FMD infection and spread. Not Available |
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Date |
2023-05-12T04:57:57Z
2023-05-12T04:57:57Z 2008-09-23 |
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Type |
Research Paper
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Identifier |
Joyappa DH, Sasi S, Ashok KC, Reddy GR, Suryanarayana VV. The plasmid constructs producing shRNA corresponding to the conserved 3D polymerase of Foot and Mouth Disease virus protects guinea pigs against challenge virus. Vet Res Commun. 2009 Mar;33(3):263-71. doi: 10.1007/s11259-008-9174-3. Epub 2008 Sep 23. PMID: 18810649.
1573-7446 http://krishi.icar.gov.in/jspui/handle/123456789/77182 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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