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A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range

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Title A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range
Not Available
 
Creator M Hosamani
S Gopinath
B P Sreenivasa
S Behera
S H Basagoudanavar
A Boora
D P Bora
P Deka
V Bhanuprakash
R K Singh
A Sanyal
K Weerdmeester
A Dekker
 
Subject blocking ELISA
Foot-and-mouth disease
Monoclonal antibody
 
Description Not Available
Large-scale monitoring of foot-and-mouth disease (FMD) in livestock is imperative in an FMD control program. Detection of antibodies against non-structural proteins (NSP) of FMD virus (FMDV) is one of the best tools to estimate the prevalence of past infection; availability of such a well-validated test is therefore essential. Using a FMDV 3B protein-specific monoclonal antibody, we have developed a new NSP antibody blocking ELISA (10H9 bELISA) and validated it on large panels of sera from different susceptible species. The diagnostic sensitivity of the ELISA was 95% with a specificity of 98%, similar to the values found using a commercial kit (PrioCHECK FMD NS test). The 10H9 bELISA can be used in a broad range of FMD susceptible species making it a very useful tool in monitoring the foot-and-mouth disease control programs by detection of virus circulation in the vaccinated populations.
Not Available
 
Date 2023-05-12T05:38:24Z
2023-05-12T05:38:24Z
2022-09-12
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77192
 
Language English
 
Relation Not Available;
 
Publisher Applied Microbiology and Biotechnology