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Evaluation of stability of live attenuated camelpox vaccine stabilized with different stabilizers and reconstituted with various diluents

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Title Evaluation of stability of live attenuated camelpox vaccine stabilized with different stabilizers and reconstituted with various diluents
 
Creator Prabhu M, Bhanuprakash V, Venkatesan G, Yogisharadhya R, Bora DP, Balamurugan V.
 
Subject Camelpox virus; Thermostability; Vaccine
 
Description In this study, thermostability of a Vero cell attenuated live camelpox vaccine under conventional lyophilization conditions has been evaluated. Three stabilizers were used separately for freeze-drying the vaccine and the stability of the vaccine, both in freeze-dried and reconstituted forms at different temperatures was assessed. The study revealed that the camelpox vaccine lyophilized with TAA stabilizer found superior with a shelf life of 44 months, 227 days, 22 days and 20 days at 4, 25, 37 and 45 °C, respectively followed by LS stabilizer. In terms of half-life, TAA stabilizer proved better followed by LS and BUGS stabilizers at all temperatures except at 25 °C in which LS found relatively superior. Among the four diluents viz. 1x PBS (phosphate buffered saline, pH 7.4), 0.85% NaCl, distilled water and 1 M MgSO4, PBS was a better diluent followed by 0.85% NaCl. Both the diluents maintained the infectivity titer more than the minimum effective dose (3 log10TCID50 with a maximum titre of 6.53 log10TCID50 in both the diluents) for 60 h at 37 and 45 °C. However, 1 M MgSO4 found less suitable for camelpox vaccine dilution. The study indicates that the TAA and 1× PBS are the choice of stabilizer and diluent, respectively for camelpox vaccine.
 
Date 2023-05-12T09:37:59Z
2023-05-12T09:37:59Z
2014-05-01
 
Type Research Paper
 
Identifier Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77215
 
Language English
 
Publisher Elsevier