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Expression and purification of recombinant type IV fimbrial subunit protein of Pasteurella multocida serogroup B:2 in Escherichia coli.
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View Archive Info| Field | Value | |
| Title |
Expression and purification of recombinant type IV fimbrial subunit protein of Pasteurella multocida serogroup B:2 in Escherichia coli.
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| Creator |
Shivachandra SB, Yogisharadhya R, Ahuja A, Bhanuprakash V.
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| Subject |
P multocida, E Coli, Type IV fimbriae, Expression, Purification
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| Description |
Pasteurella multocida serogroup B:2, a causative agent of haemorrhagic secpticaemia (HS) in cattle and buffalo especially in tropical regions of Asia and African countries, is known to possess a type IV fimbriae (pili) as one of the virulent factors. In the present study, ptfA gene encoding for type IV fimbrial subunit of P. multocida serogroup B:2 (strain p52), an Indian HS vaccine strain, has been cloned and over-expressed in recombinant Escherichia coli. The recombinant type IV fimbrial subunit protein (∼31 kDa) including N-terminus histidine tag was purified under denaturing condition and confirmed by western blotting. A homology model of HS causing P. multocida serogroup B:2 fimbrial subunit has also been discussed. The study indicated the potential possibilities to use the recombinant fimbrial protein in developing HS subunit vaccine along with suitable adjuvant.
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| Date |
2023-05-12T10:13:29Z
2023-05-12T10:13:29Z 2012-12-01 |
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| Type |
Research Paper
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| Identifier |
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77227 |
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| Language |
English
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| Publisher |
Elsevier
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