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Mutation of VP2 capsid protein of Foot-and-mouth disease virus and its expression in the insect cell system for increased thermostability

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Title Mutation of VP2 capsid protein of Foot-and-mouth disease virus and its expression in the insect cell system for increased thermostability
Not Available
 
Creator V.K. Ganji
J.K. Biswal
S.H. Basagoudanavar
P. Saravanan
M. Hosamani
Aniket Sanyal
H.J. Dechamma
 
Subject Mutation
FMD
insect cell
 
Description Not Available
FMD is an economically devastating livestock disease of global importance. The disease is caused by FMD virus of genus
Aphthovirus of family picornaviridae. It affects both domestic and wild cloven-hoofed animals. Many alternate vaccine strategies
such as peptide vaccines, DNA vaccines, viral-vectored vaccine, VLPs are attempted to date. The limited success of alternate
strategies was attributed to either poor immune response or instability of antigen during storage. The FMDV capsid is reported to
be thermo- and acid-labile. In this study, we report engineering and evaluation of FMDV capsid for enhanced thermostability.
In this study, five mutations (TS1, TS2, TS3, TS4 and TS5) with a single amino acid change were introduced in VP2 region, by
PCR-mediated site-directed mutagenesis and generated recombinant bacmids by transposition method, transfected in insect cells
to produce recombinant baculovirus expressing FMDV empty capsids. The expressed VLPs were analyzed for antigenic
thermostability, by storing the samples at -20oC, 4oC, 24oC and 37oC and subjecting to s-ELISA at 15 days interval. Purified
VLPs were also investigated based on Tm from DSF stability assay after preheating at 37oC for 1hr, 45oC and 56oC for 30 min
and varying pH conditions like 6, 7 and 8. The expressed protein purified by 15 to 60% sucrose gradient, on analysis by TEM
showed the intact empty capsid particles of ~25nm. The s-ELISA of stored samples revealed the consistency in reactivity of TS1,
TS2 and TS3 samples stored at -20oC, 4oC, 24oC and 37oC for more than 75 days, while the drop in antigen reactivity was
noticed for wild-type after 30 days and for TS5 after 45 days when stored at the different temperatures. TS4 showed stability
when stored at -20oC, 4oC for more than 75 days but the sudden decrease in antigen reactivity was seen after 45 days at 24oC and
after 30 days at 37oC. In DSF stability assay, TS3 exhibited a constant high Tm after heating at 37oC for 1hr, 45oC for 30min and
under pH conditions 6, 7 and 8 in comparison to other mutants and wild-type. However, the complete dissociation of capsid was
seen in all mutants and wild-type when heated at 56oC for 30 min. TS3 capsids exhibited relatively more stability compared to all
other mutant capsids. Further studies evaluating TS3 mutant capsids in eliciting a protective immune response are needed to
investigate its potential as a vaccine candidate.
Not Available
 
Date 2023-05-12T10:59:33Z
2023-05-12T10:59:33Z
2017-12-01
 
Type Presentation
 
Identifier Ganji, V.K., Biswal, J.K., Basagoudanavar, S.H., Saravanan, P., Hosamani, M., Sanyal, A. and Dechamma, H.J. Mutation of VP2 capsid protein of Foot-and-mouth disease virus and its expression in the insect cell system for increased thermostability
ISSN 2560-8304
http://krishi.icar.gov.in/jspui/handle/123456789/77250
 
Language English
 
Relation Not Available;
 
Publisher Not Available