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DNA methylation analysis of porcine mammary epithelial cells reveals differentially methylated loci associated with immune response against Escherichia coli challenge

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Title DNA methylation analysis of porcine mammary epithelial cells reveals differentially methylated loci associated with immune response against Escherichia coli challenge
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Creator Basavaraj Sajjanar
Nares Trakooljul
Klaus Wimmers
Siriluck Ponsuksili
 
Subject DNA methylations, Coliform mastitis, Immune response, CpG islands
 
Description Not Available
Background: Epigenetic changes such as cytosine (CpG) DNA methylations regulate gene expression patterns in response to environmental cues including infections. Microbial infections induce DNA methylations that play a potential role in modulating host-immune response. In the present study, we sought to determine DNA methylation
changes induced by the mastitis causing Escherichia coli (E. coli) in porcine mammary epithelial cells (PMEC). Two time points (3 h and 24 h) were selected based on specific transcriptomic changes during the early and late immune responses, respectively.
Results: DNA methylation analysis revealed 561 and 898 significant (P < 0.01) differentially methylated CpG sites at 3 h and 24 h after E. coli challenge in PMEC respectively. These CpG sites mapped to genes that have functional roles in
innate and adaptive immune responses. Significantly, hypomethylated CpG sites were found in the promoter regions of immune response genes such as SDF4, SRXN1, CSF1 and CXCL14. The quantitative transcript estimation indicated higher expression associated with the DNA CpG methylation observed in these immune response genes. Further, E. coli challenge significantly reduced the expression levels of DNMT3a, a subtype of de novo DNA methylation enzyme in PMEC indicating the probable reason for the hypomethylation observed in the immune response genes.
Conclusions: Our study revealed E. coli infection induced DNA methylation loci in the porcine genome. The differentially methylated CpGs were identified in the regulatory regions of genes that play important role in immune response. These results will help to understand epigenetic mechanisms for immune regulation during coliform mastitis in pigs.
 
Date 2023-05-16T04:26:13Z
2023-05-16T04:26:13Z
2019-07-31
 
Type Research Paper
 
Identifier Sajjanar, B., Trakooljul, N., Wimmers, K. et al. DNA methylation analysis of porcine mammary epithelial cells reveals differentially methylated loci associated with immune response against Escherichia coli challenge. BMC Genomics 20, 623 (2019). https://doi.org/10.1186/s12864-019-5976-7
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http://krishi.icar.gov.in/jspui/handle/123456789/77454
 
Language English
 
Relation Not Available;
 
Publisher Springer Nature