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Standardization of protocol for in-vitro shoot tip grafting in Kinnow mandarin (Citrus deliciosa)

Indian Agricultural Research Journals

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Title Standardization of protocol for in-vitro shoot tip grafting in Kinnow mandarin (Citrus deliciosa)
 
Creator KUMAR, RAJ
KAUL, M K
SAXENA, S N
SINGH, A K
KHADDA, B S
 
Subject Apical meristem, Kinnow mandarin, Plant growth regulators, Seedlings, Shoot tip grafting
 
Description An experiment to standardize the rootstocks and PGRs for success and survival of in-vitro shoot tip grafting in Kinnow mandarin (Citrus deliciosa) was conducted during the year of 2007-08 at Department of Horticulture, Agricultural Research Station, Sriganganagar, Rajasthan. For this, in-vitro generated and 2-3 weeks etiolated old seedlings of cleopatra, rough lemon and carrizo were used as rootstocks. The seedlings were grafted by shoot tips measuring = 1 mm in length containing apical meristem and one or two leaf primordial which were excised from in-vitro generated shoots and grafted on rootstocks. Among different rootstocks used, twelve days old seedlings of Carrizo recorded the maximum success (56.60 %) and length of shoot (1.43 cm). All the concentrations of the PGRS tried to accelerate the growth of new shoot and survival per cent of STG, BAP @ 1.0 mg/l was found to be best in minimizing the time taken to bud break (12.12 days) and recorded maximum shoot length (3.10 cm). The results of the study revealed that the maximum survival (66.90 %) was observed with 2, 4-D @ 3.0 mg/l in all the PGRs tried for growth promotion of scion shoots. It is also revealed from the study that the 90 % grafted plants survival in greenhouse at after 45 days after shoot tip grafting. Based on the observations, the Carrizo as rootstocks, BAP and 2, 4-D were found to be the best protocol for shoot tip grafting in Kinnow mandarin.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2014-11-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/44641
 
Source The Indian Journal of Agricultural Sciences; Vol 84, No 11 (2014)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/44641/19584
 
Rights Copyright (c) 2014 The Indian Journal of Agricultural Sciences