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Cell culture adapted sheeppox virus as a challenge virus for potency testing of sheeppox vaccine.

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Title Cell culture adapted sheeppox virus as a challenge virus for potency testing of sheeppox vaccine.
 
Creator Hosamani M, Bhanuprakash V, Kallesh DJ, Balamurugan V, Pande A, Singh RK.
 
Subject Cell Culture, Sheeppox Virus, Challenge Virus
 
Description Sheeppox virus from an outbreak of sheeppox that occurred in Srinagar (Jammu and Kashmir, India) in 2000 was isolated by inoculation of susceptible sheep and further re-isolated in cell culture. The field virus, adapted to grow in lamb testes culture, was evaluated for its potential use as challenge virus in potency testing of sheeppox vaccine currently in use. The virus (passage 6) produced severe disease in susceptible sheep when inoculated subcutaneously with a dose of 106.2 TCID50. The virus identity was confirmed by PCR, sequencing of P32 gene and species-specific signature residues identified in deduced aa sequence of the gene. The virus was successfully evaluated for its virulence using two batches of sheep pox vaccines. Use of this field virus enables consistent potency experiments of sheeppox vaccines avoiding use of animals for its propagation and titration.
 
Date 2023-05-16T04:43:23Z
2023-05-16T04:43:23Z
2008-10-01
 
Type Research Paper
 
Identifier http://krishi.icar.gov.in/jspui/handle/123456789/77461
 
Language English
 
Publisher Council for Scientific and Industrial Research [NISCAIR]