Effect of Mito-Tempo Incorporated Semen Extender On Physico-Morphological Attributes and Functional Membrane Integrity of Frozen Thawed Buffalo Spermatozoa
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Title |
Effect of Mito-Tempo Incorporated Semen Extender On Physico-Morphological Attributes and Functional Membrane Integrity of Frozen Thawed Buffalo Spermatozoa
Not Available |
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Creator |
Kumar Abhishek
Ghosh Subrata Kumar Katiyar Rahul Rautela Rupali Bisla, Amarjeet Ngou Athanas Alex Pande Megha Srivastava Neeraj Bhure SK |
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Subject |
mito-TEMPO
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Description |
Not Available
Background: Sperm mitochondria are the major site of reactive oxygen species (ROS) production and excess production during freezing-thawing process inflicts oxidative damages to spermatozoa. Buffalo spermatozoa are more prone to oxidative damage due to inherently more polyunsaturated fatty acids and low cholesterol to phospholipids ratio in the plasma membrane. A mitochondrial targeted antioxidant, Mito-TEMPO was used in this study. Objective: To study the effect of Mito-TEMPO incorporated semen extender on the post-thaw semen quality in buffalo. Materials and methods: A total of 18 ejaculates from three murrah buffalo bulls with ≥70% individual progressive motility were utilized for the study. Each semen sample was equally divided and extended with five groups: Group I (Control, without Mito-TEMPO addition); Group II (10 µM Mito-TEMPO); Group III (50 µM Mito-TEMPO); Group IV (100 µM Mito-TEMPO); Group V (500 µM Mito-TEMPO) to have 80×106 progressive motile sperm/mL of extender, filled and sealed in French mini straws (0.25 mL) and frozen following equilibration. The effect of Mito-TEMPO was assessed at fresh/post-dilution and post-thaw stages by evaluating physico-morphological attributes and functional membrane integrity such as hypo-osmotic swelling test (HOST). Results: Initial progressive motility, viability, acrosomal integrity and HOS response was significantly (p Not Available |
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Date |
2023-05-16T10:19:00Z
2023-05-16T10:19:00Z 2021-04-01 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77507 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Cryo Letters
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