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Effect of Mito-Tempo Incorporated Semen Extender On Physico-Morphological Attributes and Functional Membrane Integrity of Frozen Thawed Buffalo Spermatozoa

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Title Effect of Mito-Tempo Incorporated Semen Extender On Physico-Morphological Attributes and Functional Membrane Integrity of Frozen Thawed Buffalo Spermatozoa
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Creator Kumar Abhishek
Ghosh Subrata Kumar
Katiyar Rahul
Rautela Rupali
Bisla, Amarjeet
Ngou Athanas Alex
Pande Megha
Srivastava Neeraj
Bhure SK
 
Subject mito-TEMPO
 
Description Not Available
Background: Sperm mitochondria are the major site of reactive oxygen species (ROS) production and excess production during freezing-thawing process inflicts oxidative damages to spermatozoa. Buffalo spermatozoa are more prone to oxidative damage due to inherently more polyunsaturated fatty acids and low cholesterol to phospholipids ratio in the plasma membrane. A mitochondrial targeted antioxidant, Mito-TEMPO was used in this study.

Objective: To study the effect of Mito-TEMPO incorporated semen extender on the post-thaw semen quality in buffalo.

Materials and methods: A total of 18 ejaculates from three murrah buffalo bulls with ≥70% individual progressive motility were utilized for the study. Each semen sample was equally divided and extended with five groups: Group I (Control, without Mito-TEMPO addition); Group II (10 µM Mito-TEMPO); Group III (50 µM Mito-TEMPO); Group IV (100 µM Mito-TEMPO); Group V (500 µM Mito-TEMPO) to have 80×106 progressive motile sperm/mL of extender, filled and sealed in French mini straws (0.25 mL) and frozen following equilibration. The effect of Mito-TEMPO was assessed at fresh/post-dilution and post-thaw stages by evaluating physico-morphological attributes and functional membrane integrity such as hypo-osmotic swelling test (HOST).

Results: Initial progressive motility, viability, acrosomal integrity and HOS response was significantly (p
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Date 2023-05-16T10:19:00Z
2023-05-16T10:19:00Z
2021-04-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77507
 
Language English
 
Relation Not Available;
 
Publisher Cryo Letters