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A novel recombinant Meq protein based dot-ELISA for rapid and confirmatory diagnosis of Marek’s disease induced lymphoma in poultry

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Title A novel recombinant Meq protein based dot-ELISA for rapid and confirmatory diagnosis of Marek’s disease induced lymphoma in poultry
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Creator Kumar, M Asok
Barathidasan, R
Palanivelu, M
Singh, SD
Wani, Mohd Yaqoob
Malik, Yashpal Singh
Singh, R
Dhama, K
 
Subject Diagnosis; Dot-ELISA; Lymphoma; Marek’s disease virus; Meq oncoprotein; Poultry.
 
Description Not Available
Marek's disease (MD), is an economically important virus disease of poultry throughout the world. In this study, we for the first time reports development of a novel dot enzyme-linked immunosorbent assay (dot-ELISA) for the confirmatory diagnosis of lymphoma caused by Marek's Disease Virus (MDV). Suspected lymphoma tissue extracts from the diseased birds were used for the Meq oncoprotein antigen detection, which is expressed specifically in MDV lymphomas. Recombinant Meq oncoprotein was expressed using Expresso™ Rhamnose Sumo Cloning and Expression system and the hyperimmune serum was raised against it, which was used later while developing dot-ELISA. The dot-ELISA exhibited higher specificity (92%) in diagnosing MD lymphomas as compared to conventional PCR (40%), where later assay is unable to differentiate disease development (lymphoma) and/or infection. The developed dot-ELISA proved to be a specific, rapid and inexpensive technique detecting MDV lymphomas in poultry. Of the note, this new assay could be opted as a valuable diagnostic tool in the resource poor countries andcould further be used to differentiate from other tumor causing viruses in poultry.
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Date 2023-05-16T10:26:30Z
2023-05-16T10:26:30Z
2016
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77514
 
Language English
 
Relation Not Available;
 
Publisher Elsevierre