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Gain of function studies on predicted host receptors for white spot virus.

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Title Gain of function studies on predicted host receptors for white spot virus.
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Creator Not Available
 
Subject White spot syndrome virus WSSV Shrimp disease Viral pathogenesis Host-pathogen interaction
 
Description Not Available
Three decades after its first outbreak, the shrimp white spot virus (WSV) is still a global cause of concern due to
considerable losses and lack of effective control measures. Several candidate host receptor proteins have been
identified, but the pathogenesis is not clearly understood, although the key role of the WSV envelope protein
VP28 in virus internalization is established. Here, protein-protein docking is applied to evaluate the interaction
of VP28 trimeric extracellular region with four host (Penaeus monodon) receptors reported earlier, Rab7 GTPase (PmRab7), glucose transporter 1 (PmGLUT1), C-type lectin (PmCTL) and calreticulin (PmCRT). The stability of predicted complexes evaluated in terms of binding energy per unit buried surface area ranged from -8.46 to -11.82 cal mol -1/A2 , which is not sufficient for functional interaction. Nevertheless, each of these host proteins
was tested by a gain-of-function approach by observing their ability to make a fish cell line permissive to the
shrimp WSV. Full-length expression constructs of the four receptors were transfected into SSN1 snakehead fish
cells that are non-permissive to WSV. Transfected SSN1 cells and WSV permissive insect Sf9 cells were challenged
with purified WSV. After 24 h, the presence of receptor transcripts was confirmed in the treated SSN1 cells, and
not in the non-transfected SSN1 cells. Further, vp28 transcript was detected in Sf9 cells, but not in any of the
treated SSN1 cells, indicating that none of the receptors were singly sufficient to make SSN1 cells permissive to
WSV, even though PmRab7 was a strong candidate that alone showed >85% protection in virus neutralization
experiments. For the other 3 candidates, previous reports predicted the involvement of co-receptors, which is
confirmed here by their inability to act singly.
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Date 2023-05-16T02:57:20Z
2023-05-16T02:57:20Z
2022-09-02
 
Type Journal
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/77300
 
Language English
 
Relation Not Available;
 
Publisher Elsevier