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Occurrence, pathogroup distribution and virulence genotypes of Escherichia coli from fresh seafood.

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Title Occurrence, pathogroup distribution and virulence genotypes of Escherichia coli from fresh seafood.
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Subject Seafood Escherichia coli Pathogroup Fecal contamination Risk assessment Serotype
 
Description Not Available
Seafood contamination with pathogenic Escherichia coli is a significant public health threat in developing
countries. In this study, the prevalence of different pathogroups of E. coli was investigated in fresh seafood. A
total of 78 samples comprising of finfish (41) and shellfish (37) were analyzed using multiple selective-
enrichment-isolation protocols. From all samples, 639 E. coli were isolated and tested for the presence of
pathogroup-specific virulence-associated genes by polymerase chain reaction (PCR). Based on the distribution of virulence genes, 118 isolates belonged to enterohemorrhagic E. coli (EHEC) group, 25 to enteropathogenic E. coli (EPEC), 20 to enterotoxigenic E. coli (ETEC), 16 to enteroaggregative E. coli (EAEC) and 1 to enteroinvasive E. coli (EIEC). Fifty-five samples of seafood, 29 finfish and 26 shellfish, were found contaminated with pathogenic E. coli. Among different enrichment broth-selective agar combinations used for the recovery of pathogenic E. coli from seafood, enrichment in Tryptone Phosphate (TP) broth followed by isolation on sorbitol MacConkey (SMAC) agar performed superior to others. The study highlights the importance of using selective isolation and genotyping protocols to identify human pathogenic E. coli in seafood in regions with extensive anthropogenic contamination.
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Date 2023-05-16T03:02:45Z
2023-05-16T03:02:45Z
2021-11-12
 
Type Journal
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/77318
 
Language English
 
Relation Not Available;
 
Publisher Elsevier