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Molecular cloning, characterization and constitutive expression analysis of TLR4 gene in Pangasianodon hypophthalmus

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Title Molecular cloning, characterization and constitutive expression analysis of TLR4 gene in Pangasianodon hypophthalmus
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Creator Not Available
 
Subject Fish, Pangasianodon hypophthalmus, phTLR4, RT-PCR, Tissue distribution, TLR4
 
Description Not Available
Toll like receptor (TLR) signalling pathway plays a central role in inflammatory responses through the
recognition of PAMPs and DAMPs. Toll-like receptor 4 (TLR4) is well known for the recognition of LPS of
gram-negative bacteria leading to production of inflammatory cytokines. Modulation of a TLR pathway gene in
different tissues indicates the functional importance of the tissues in innate immunity. The aim of this study was
to clone and characterize TLR4 and evaluate its constitutive tissue distribution in Pangasianodon hypophthalmus, a commercially important fish species. The study was carried out at ICAR-CIFE, Mumbai during 2017. Partial nucleotide and amino acid sequences of TLR4 were identified in P. hypophthalmus and termed as phTLR4. Bioinformatics analysis of partial sequence revealed four putative LRR domains characteristic of TLRs. Nucleotide and amino acid sequences showed varying degree of identity with TLR4 sequences from other fish species in BLASTn and BLASTp analysis. Phylogenetic analysis placed the phTLR4 close to Ictalurus punctatus. TLR4 was found to be constitutively expressed in all the tissues studied. The significantly highest expression was recorded in intestine and spleen. The lowest expression was in muscle, heart and skin. House-keeping gene ß-actin was used as an internal control. The outcome of this research will be helpful for studying mRNA expression levels of phTLR4 under different conditions in different tissues of Pangasianodon hypophthalmus.
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Date 2023-05-16T03:11:02Z
2023-05-16T03:11:02Z
2022-08-28
 
Type Journal
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/77345
 
Language English
 
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Publisher Not Available