Molecular cloning and expression profiling of insulin-like growth factor 2 and IGF-binding protein 6 in Clarias magur (Hamilton 1822).
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Title |
Molecular cloning and expression profiling of insulin-like growth factor 2 and IGF-binding protein 6 in Clarias magur (Hamilton 1822).
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Creator |
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Subject |
Growth-axis Pathway, IGFBP6, Fast-growing, RACE-PCR, Relative Expression
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Description |
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Growth is an important trait in aquaculture and the major genes that regulate it are Insulin-like growth factors (IGFs) and IGF binding proteins (IGFBPs). In this study, the full-length coding sequences of IGF2 and IGFBP6 genes in the Indian catfish Clarias magur were cloned and characterized. The full-length cDNA sequences of IGF2 and IGFBP6 were 885 bp (ORF 642 bp) and 928 bp (ORF 600 bp), encoding 213 and 199 amino acids, respectively. Bioinformatics analyses revealed that the magur IGF2 and IGFBP6 proteins are hydrophilic and secretory in nature. Sequence alignment with other teleosts and mammalian orthologues shows conservation of the functional domains. Gene expression analysis in 6 individuals each of high (298 ± 5.0 g) and low (210 ± 6.0 g) growth performing families showed significantly (p Not Available |
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Date |
2023-05-16T04:06:35Z
2023-05-16T04:06:35Z 2022-06-17 |
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Type |
Journal
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Identifier |
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Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77398 |
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Language |
English
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Relation |
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Publisher |
Taylor&Francis Online
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