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Pathogenesis of Aeromonas caviae in Clarias magur.

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Title Pathogenesis of Aeromonas caviae in Clarias magur.
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Creator Not Available
 
Subject Clarias magur Aeromonas caviae 16S rRNA gene Virulence genes Necrosis
 
Description Not Available
Aeromonas spp. is a pathogenic bacteria that potentially cause infection in farmed fish, including Catfishes. In the
present study, dominant bacteria were isolated from diseased Clarias magur and tentatively named BLBM-05.
Based on morphological, physiological, and biochemical features as well as 16S rRNA gene sequence and gyrB
gene sequences (Gen Bank accession number: MT973994.1 and MZ398017.1), the bacteria in the isolate was
found to be Aeromonas caviae. Further, the isolate was screened for five known virulence genes, namely β-he-
molysin, lafA, exu, ompA1 and ascV. Among them, three virulence genes related to pathogenicity, including
aerolysin (aer), outer membrane protein (ompA1), lateral flagella (lafA), were identified in the A. caviae isolate.
The median lethal dosage (LD50) of the BLBM-05 isolate for magur was determined as 1.53x106 CFU/mL. The
histopathological analysis showed that the BLBM-05 isolate induced considerable histological lesions in the
magur fish, including necrosis, hemolysis of erythrocytes, myolysis, hemorrhage, and desquamation in the in-
testinal tissue, tissue loosening, and infiltration of inflammatory cells. Drug sensitivity test showed that the
isolate was susceptible to Gentamicin, Ceftazidine, Ceftrioxone, Amikacin, Tetracycline, Meropener and
Oxytetracycline. The present results provide a scientific basis to identify A. caviae further, a line of treatment for
magur infected by this pathogen.
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Date 2023-05-16T04:08:04Z
2023-05-16T04:08:04Z
2022-06-27
 
Type Journal
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/77409
 
Language English
 
Relation Not Available;
 
Publisher Elsevier