Malate synthase contributes to the survival of Salmonella Typhimurium against nutrient and oxidative stress conditions
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Title |
Malate synthase contributes to the survival of Salmonella Typhimurium against nutrient and oxidative stress conditions
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Creator |
Ratanti Sarkhel
Shekhar Apoorva Swagatika Priyadarsini Hari Balaji Sridhar Sanjeev Kumar Bhure Manish Mahawar |
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Subject |
Malate synthase
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Description |
Not Available
To survive and replicate in the host, S. Typhimurium have evolved several metabolic pathways. The glyoxylate shunt is one such pathway that can utilize acetate for the synthesis of glucose and other biomolecules. This pathway is a bypass of the TCA cycle in which CO2 generating steps are omitted. Two enzymes involved in the glyoxylate cycle are isocitrate lyase (ICL) and malate synthase (MS). We determined the contribution of MS in the survival of S. Typhimurium under carbon limiting and oxidative stress conditions. The ms gene deletion strain (∆ms strain) grew normally in LB media but failed to grow in M9 minimal media supplemented with acetate as a sole carbon source. However, the ∆ms strain showed hypersensitivity (p < 0.05) to hypochlorite. Further, ∆ms strain has been significantly more susceptible to neutrophils. Interestingly, several folds induction of ms gene was observed following incubation of S. Typhimurium with neutrophils. Further, ∆ms strain showed defective colonization in poultry spleen and liver. In short, our data demonstrate that the MS contributes to the virulence of S. Typhimurium by aiding its survival under carbon starvation and oxidative stress conditions. This piece of work was funded by the Department of Biotechnology, India (Grant No.: BT/PR13689/ BRB/10/1399/2015) and NASF, ICAR, India (Grant No.: NFBSFARA/BS-3012/2012-13). The funders have no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. We thank our Director, ICAR-Indian Veterinary Research Institute (IVRI) for providing the necessary facilities. Ratanti Sarkhel acknowledges the support from ICAR and Indian Council of Medical Research (ICMR-SRF), India. |
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Date |
2023-05-17T04:46:28Z
2023-05-17T04:46:28Z 2022-09-25 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77555 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Natureres
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