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Malate synthase contributes to the survival of Salmonella Typhimurium against nutrient and oxidative stress conditions

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Title Malate synthase contributes to the survival of Salmonella Typhimurium against nutrient and oxidative stress conditions
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Creator Ratanti Sarkhel
Shekhar Apoorva
Swagatika Priyadarsini
Hari Balaji Sridhar
Sanjeev Kumar Bhure
Manish Mahawar
 
Subject Malate synthase
 
Description Not Available
To survive and replicate in the host, S. Typhimurium have evolved several metabolic pathways. The glyoxylate shunt is one such pathway that can utilize acetate for the synthesis of glucose and other biomolecules. This pathway is a bypass of the TCA cycle in which CO2 generating steps are omitted. Two enzymes involved in the glyoxylate cycle are isocitrate lyase (ICL) and malate synthase (MS). We determined the contribution of MS in the survival of S. Typhimurium under carbon limiting and oxidative stress conditions. The ms gene deletion strain (∆ms strain) grew normally in LB media but failed to grow in M9 minimal media supplemented with acetate as a sole carbon source. However, the ∆ms strain showed hypersensitivity (p < 0.05) to hypochlorite. Further, ∆ms strain has been significantly more susceptible to neutrophils. Interestingly, several folds induction of ms gene was observed following incubation of S. Typhimurium with neutrophils. Further, ∆ms strain showed defective colonization in poultry spleen and liver. In short, our data demonstrate that the MS contributes to the virulence of S. Typhimurium by aiding its survival under carbon starvation and oxidative stress conditions.
This piece of work was funded by the Department of Biotechnology, India (Grant No.: BT/PR13689/ BRB/10/1399/2015) and NASF, ICAR, India (Grant No.: NFBSFARA/BS-3012/2012-13). The funders have no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. We thank our Director, ICAR-Indian Veterinary Research Institute (IVRI) for providing the necessary facilities. Ratanti Sarkhel acknowledges the support from ICAR and Indian Council of Medical Research (ICMR-SRF), India.
 
Date 2023-05-17T04:46:28Z
2023-05-17T04:46:28Z
2022-09-25
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/77555
 
Language English
 
Relation Not Available;
 
Publisher Natureres