Optimization of dissolved oxygen levels in extender prevents development of cryocapacitation like changes, oxidative stress and augments zona binding capacity of crossbred bull spermatozoa
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Optimization of dissolved oxygen levels in extender prevents development of cryocapacitation like changes, oxidative stress and augments zona binding capacity of crossbred bull spermatozoa
Not Available |
|
Creator |
Abdul‐Rahman Mustapha
Subrata Kumar Ghosh Jai Kishan Prasad Rahul Katiyar Abhishek Kumar Beigh Y Amin Sadhan Bag Sanjeev K Bhure Guttula Taru Sharma Med Ram Verma |
|
Subject |
crossbred bull
dissolved oxygen oxidative stress semen zona binding |
|
Description |
Not Available
The present study was undertaken to determine the efficacy of partial deoxygenation of extender at constant temperature (35°C) in freezability of crossbred bull semen. The dissolved oxygen (DO) levels were reduced by the use of newly developed technique of nitrogen effervescence at a flow rate of 2-3 bubbles per second. Four different levels of oxygen in semen extender, that is 11.7, 2, 4 and 8 ppm as control (Group-I), Group-II, Group-III and Group-IV, respectively, were used to assess the effect of partial deoxygenation on semen quality parameters. The 4 ppm level of DO resulted in higher (p < 0.05) progressive motility in comparison with non-treated group at post-thaw stage, whereas reduction up to 2 ppm resulted in drastic fall in motility. Oxidative stress status revealed low superoxide dismutase (SOD) and total antioxidant capacity (TAC) in Group-II, whereas higher (p < 0.05) SOD and TAC activities were observed in Group-III in comparison with non-treated group at pre-freeze and post-thaw stages. The sperm-zona binding at 4 ppm level of DO was significantly higher than control group, 2 and 8 ppm levels of DO. In conclusion, reduction of DO in the extender up to 4 ppm reduced oxidative stress and improved in vitro fertility of crossbred bull spermatozoa. Not Available |
|
Date |
2023-05-17T04:05:40Z
2023-05-17T04:05:40Z 2021-03-01 |
|
Type |
Research Paper
|
|
Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77550 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Wileyre
|
|