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Antibiotic Resistance, Virulence Gene and Molecular Profiles of Shiga Toxin Producing Escherichia coli Isolates from Diverse Sources in Calcutta, India

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Title Antibiotic Resistance, Virulence Gene and Molecular Profiles of Shiga Toxin Producing Escherichia coli Isolates from Diverse Sources in Calcutta, India
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Creator Khan A, Das SC, Ramamurthy T, Sikdar A, Khanam J, Yamasaki S, Takeda Y and Nair G. Balakrish
 
Subject Antibiotic resistance, virulence gene, molecular typing, Escherichia coli, Calcutta, India
 
Description Work done under the extramural fund from ICMR, New Delhi in collaboration with ICMR-National Institute of Cholera and Enteric Diseases (NICED), Kolkata
Antibiotic resistance, virulence gene, and molecular profiles of Shiga toxin-producing Escherichia coli (STEC) non-O157 strains isolated from human stool samples, cow stool samples, and beef samples over a period of 2 years in Calcutta, India, were determined. Resistance to one or more antibiotics was observed in 49.2% of the STEC strains, with some of the strains exhibiting multidrug resistance. The dominant combinations of virulence genes present in the strains studied were stx(1) and stx(2) (44.5% of strains) and stx(1), stx(2), and hlyA (enterohemorrhagic E. coli hemolysin gene) (19% of strains). Only 6.4% of the STEC strains harbored eae. The diversity of STEC strains from various sources was assessed by random amplification of polymorphic DNA (RAPD). STEC strains that gave identical or nearly similar DNA fingerprints in RAPD-PCR and had similar virulence genotypes were further characterized by pulsed-field gel electrophoresis (PFGE). Identical RAPD and PFGE profiles were observed in four sets of strains, with each set comprising two strains. There was no match in the RAPD and PFGE profiles between strains of STEC isolated from cows and those isolated from humans. It appears that the clones present in bovine sources are not transmitted to humans in the Calcutta setting although these strains showed evolutionary relatedness. Maybe for this reason, STEC has still not become a major problem in India.
ICMR New Delhi
 
Date 2023-05-18T05:09:26Z
2023-05-18T05:09:26Z
2002-01-01
 
Type Article
 
Identifier Khan A, Das SC, Ramamurthy T, Sikdar A, Khanam J, Yamasaki S, Takeda Y, Nair GB. Antibiotic resistance, virulence gene, and molecular profiles of Shiga toxin-producing Escherichia coli isolates from diverse sources in Calcutta, India. J Clin Microbiol. 2002 Jun;40(6):2009-15. doi: 10.1128/JCM.40.6.2009-2015.2002. PMID: 12037056; PMCID: PMC130831.
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http://krishi.icar.gov.in/jspui/handle/123456789/77578
 
Language English
 
Relation Not Available;
 
Publisher American Association of Microbiology (ASM)