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Dairy farm investigation on Shiga toxin-producing Escherichia coli (STEC) in Kolkata, India with emphasis on molecular traits of the strains.

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Title Dairy farm investigation on Shiga toxin-producing Escherichia coli (STEC) in Kolkata, India with emphasis on molecular traits of the strains.
Not Available
 
Creator 7. Das SC, Khan A, Panja P, Datta S, Sikdar A, Yamasaki S, Takeda Y, Bhattacharya SK, Ramamurthy T and Nair G Balakrish
 
Subject Shigatoxic Escherichia coli, dairy farm, molecular traits
 
Description Collaborative works with ICMR-NICED
An investigation was conducted to determine the distribution, virulence gene profile andphenotypes of Shiga toxin-producing Escherichia coli (STEC) strains within a dairy farm inKolkata, India by characterizing the STEC strains isolated from healthy dairy cow and calf stoolsamples, raw milk and farm floor swabs from July 2001 to March 2002. Primary screening bymultiplex-PCR detected stx1 and stx2, the common virulence genes of STEC, in 18.9 % of cowfaeces, 32.4 % of calf stool samples, 21.6 % of farm floor swabs and 4.5 % of raw milk samplesand viable STEC were recovered from 4.5, 9.9, 8.1 and 1.8% of the corresponding PCR-positivesamples. Strains harbouring stx1 (63.3 %) and hlyA (53.3 %) were frequently detected comparedto eae (13.3%). Most of the strains harboured similar sets of reported virulence genes commonamong isolates from diarrhoea patients. Most of the strains also exhibited multidrug resistance,sorbitol fermentation and produced enterohaemolysin. The randomly amplified polymorphicDNA–PCR (RAPD–PCR) profile of the STEC strains isolated from the farm milieu revealeddiverse banding patterns and clonal analysis demonstrated that the strains from different sourceswere not identical but showed some genetic relatedness. The study demonstrates the potential ofdairy farm for housing virulent STEC.

(3) (PDF) Dairy farm investigation on Shiga toxin-producing Escherichia coli (STEC) in Kolkata, India with emphasis on molecular characterization. Available from: https://www.researchgate.net/publication/7694281_Dairy_farm_investigation_on_Shiga_toxin-producing_Escherichia_coli_STEC_in_Kolkata_India_with_emphasis_on_molecular_characterization#fullTextFileContent [accessed Apr 18 2023].
ICMR-New Delhi
 
Date 2023-05-18T04:27:33Z
2023-05-18T04:27:33Z
2005-04-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77560
 
Language English
 
Relation Not Available;
 
Publisher Research Gate