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Direct detection of bovine herpesvirus-1 DNA from cell culture fluids using polymerase chain reaction
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View Archive Info| Field | Value | |
| Title |
Direct detection of bovine herpesvirus-1 DNA from cell culture fluids using polymerase chain reaction
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| Creator |
B P Sreenivasa
T J Rasool C Natarajan |
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| Subject |
polymerase chain reaction
bovine herpesvirus 1 genomic DNA |
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| Description |
Not Available
A pair of oligomers of 20 and 23 bp were designed for amplifying a 381 bp sequence from glycoprotein IV gene of bovine herpesvirus 1. The primer pairs were used for amplifying genomic DNA of BHV-1 directly from cell culture fluids under different experimental conditions such as, untreated cell culture fluid, thermal denaturation and proteinase K treatment in presence of detergent. The results reveal that direct thermal denaturation of cell culture fluid is sufficient to detect the virus by polymerase chain reaction. Not Available |
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| Date |
2023-05-22T06:03:13Z
2023-05-22T06:03:13Z 1996-11-01 |
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| Type |
Article
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77702 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Indian Journal of Experimental Biology
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