The M and N genes-based simplex and multiplex PCRs are better than the F or H gene-based simplex PCR for peste-des-petits-ruminants virus.
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Title |
The M and N genes-based simplex and multiplex PCRs are better than the F or H gene-based simplex PCR for peste-des-petits-ruminants virus.
Not Available |
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Creator |
A George
P Dhar B P Sreenivasa R P Singh S K Bandyopadhyay |
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Subject |
Nucleocapsid
Matrix hemagglutinin genes |
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Description |
Not Available
Nucleocapsid (N), matrix (M) and hemagglutinin (H) genes-based simplex PCRs and an N and M genes-based multiplex PCR were developed for detection of Peste-des-petits-ruminants virus (PPRV). The M gene PCR was the most sensitive, followed by N, H and an already described fusion (F) gene PCRs, as they could detect the virus in samples with titers of 101, 102, 104and 105 TCID50/ml, respectively. The multiplex PCR was as sensitive as the M gene PCR, but it had the advantage of differentiating PPRV from Rinderpest virus (RPV). Not Available |
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Date |
2023-05-22T06:07:04Z
2023-05-22T06:07:04Z 2006-01-01 |
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Type |
Article
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77706 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Acta virologica
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