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The M and N genes-based simplex and multiplex PCRs are better than the F or H gene-based simplex PCR for peste-des-petits-ruminants virus.

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Title The M and N genes-based simplex and multiplex PCRs are better than the F or H gene-based simplex PCR for peste-des-petits-ruminants virus.
Not Available
 
Creator A George
P Dhar
B P Sreenivasa
R P Singh
S K Bandyopadhyay
 
Subject Nucleocapsid
Matrix
hemagglutinin genes
 
Description Not Available
Nucleocapsid (N), matrix (M) and hemagglutinin (H) genes-based simplex PCRs and an N and M genes-based multiplex PCR were developed for detection of Peste-des-petits-ruminants virus (PPRV). The M gene PCR was the most sensitive, followed by N, H and an already described fusion (F) gene PCRs, as they could detect the virus in samples with titers of 101, 102, 104and 105 TCID50/ml, respectively. The multiplex PCR was as sensitive as the M gene PCR, but it had the advantage of differentiating PPRV from Rinderpest virus (RPV).
Not Available
 
Date 2023-05-22T06:07:04Z
2023-05-22T06:07:04Z
2006-01-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77706
 
Language English
 
Relation Not Available;
 
Publisher Acta virologica