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Development of a cell-ELISA using anti-nucleocapsid protein monoclonal antibody for the titration of peste des ipetits ruminants (PPR) vaccine virus.

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Title Development of a cell-ELISA using anti-nucleocapsid protein monoclonal antibody for the titration of peste des ipetits ruminants (PPR) vaccine virus.
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Creator J Sarkar
G Belaineh
B P Sreenivasa
R P Singh
 
Subject Peste des petits ruminants
viral disease
ELISA
 
Description Not Available
Peste des petits ruminants (PPR) is an acute and highly contagious viral disease of sheep and goats. Indian veterinary research Institute has developed a very efficacious vaccine using lineage IV virus of PPR being used under field condition. Infectivity assay and identity test of the vaccine virus are two important tests to be carried out routinely for quality assurance of PPR vaccine. We developed a monoclonal antibody based cell-ELISA for simultaneous titration and identity test of vaccine virus using 4G6 anti-nucleocapsid monoclonal antibody. Cell-ELISA developed correlated well (r=0.96) with the conventional titration technique using cytopathic effect of virus as indicator. Cell ELISA has a relative sensitivity and specificity of more than 97% compared to infectivity titration. Findings indicated that, cell-ELISA can be successfully used for infectivity and identity test of virus in vaccine samples.
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Date 2023-05-22T06:08:23Z
2023-05-22T06:08:23Z
2013-01-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77709
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases